PTPμ (PTPRM), GST-tag

Catalog #: 30053
Size: 20 µg
$375
Species: Human
Host Species: E. coli
MW: 56.2 kDa
Genbank #: NM_001105244
Tag(s): N-terminal GST-tag
a.a: 896–1175
Background:
PTPRM is a member of the protein tyrosine phosphatase family and can participate in a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. PTPRM has been shown to mediate cell-cell aggregation through the interaction with another molecule of PTPRM on an adjacent cell. PTPRM can interact with scaffolding protein RACK1/GNB2L1 and this interaction may be necessary for downstream signaling in response to cell-cell adhesion. PTPRM has been shown to be expressed in human pulmonary vascular endothelia where it directly binds to VE-cadherin and regulates both the tyrosine phosphorylation state of VE-cadherin and barrier integrity.
Description:
PTPμ (mu) (protein tyrosine phosphatase mu, also known as RPTPµ), catalytic domain, (a.a. 896-1175), (GenBank Accession No. NM_001105244) with N-terminal GST tag, MW=56.2 kDa, expressed in an E. coli expression system.
Specific Activity:
60 U/µg
Assay Conditions:
20 mM HEPES, pH 7.2, 100 mM NaCl, 2 mM EDTA, 1 mM DTT, 1 mM pNPP and 2 ng/µl PTP-mu. Assay was done at room temperature for 5 minutes, and the reaction was monitored at 405 nm continuously. Specific activity was calculated based on ε pNPP= 18,000 M-1cm-1.
Formulation:
25 mM Tris-HCl, pH 8.0, 75 mM NaCl, 0.05% Tween-20, 50% glycerol, 2 mM EDTA, 1 mM DTT, 10 mM glutathione
Format:
Aqueous buffer solution
Storage / Stability:

>12 months at -80°C.

Application(s):
Useful for the study of enzyme kinetics and regulation, to dephosphorylate target substrates and for screening inhibitors.
Reference(s):
1. S. Brady-Kalnay, et al., J. Cell Biol 1995, 130: 977.
2. T. Mourton, et al., J. Biol. Chem. 2002, 276:14896.
Warning(s):
Avoid freeze/thaw cycles
Scientific Category:
PTP/Receptor
Product Type:
Recombinant Protein
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Product Datasheets

30053_Lot121108.pdf