Mouse PTP1B(1-321)/PTPN, GST-Tag Recombinant

Catalog #

30012

$325 *

Size: 50 µg

Qty

Bulk/Custom

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Description

Murine recombinant PTP1B Protein tyrosine phosphatase, also known as PTPN1 (Genbank accession No. NM_011201.3), a.a. 1-321 (catalytic domain), with N-terminal GST-tag, MW=63.3 kDa, expressed in an E. coli expression system.

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Synonyms

PTP1B, PTPN1, Protein tyrosine phosphatase 1B, ptp

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Mouse PTP1B Activity

Product Info

Storage and Usage

Citations

Species

Mouse

Construct

PTP1B (GST-1-321) (Mouse)

Host Species/Expression System

E. coli

Purity

≥90

Format

Aqueous buffer solution

Formulation

25 mM Tris-HCl, 75 mM NaCl, pH 8.0, 0.05% Tween-20, variable glutathione, 1 mM DTT, and 50% glycerol

63 kDa

Amino Acids

1–321

Specific Activity

350 pmole/min/µg

Genbank #

NM_011201.3

Tag(s)

N-terminal GST-tag

Background

Protein tyrosine phosphatase nonreceptor, type 1 (PTPN1) is the founding member of the protein tyrosine phosphatase (PTP) family. PTPN1 catalyze the hydrolysis of the phosphate monoesters specifically from tyrosine residues. PTPN1 is known to be involved in signaling pathways that regulate a variety of cellular processes including cell growth and oncogenic transformation. PTPN1 also has been shown to act as a negative regulator of insulin signaling. PTPN1 has also been reported to dephosphorylate epidermal growth factor receptor kinase, which implicated the role of this phosphatase in cell growth control and cell response to interferon stimulation.

References

1. Frangioni et al., Cell, 1992, 68: 545.
2. M. Elchebly et al., Science 1999, 283: 1544.

Storage/Stability

At least 6 months at -80°C.

Assay Conditions

Assay was done according to PTP1B (Catalytic Domain) Colorimetric Assay Kit (BPS Bioscience #30019) with various amounts of PTP1B.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage.

Applications

Useful for the study of enzyme kinetics and regulation, to dephosphorylate target substrates and for screening inhibitors.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

PTP/Non-rec.

Regulatory Status

Research Use Only