PTP-D2/PTPN14, GST-tag

Catalog #: 30025
Size: 20 µg
$375
Species: Human
Host Species: E. coli
MW: 61 kDa
Genbank #: NM_005401
Tag(s): N-terminal GST-tag
a.a: 817–1188
Background:
Protein tyrosine phosphatase which may play a role in the regulation of lymphangiogenesis, cell-cell adhesion, cell-matrix adhesion, cell migration, cell growth and also regulates TGF-beta gene expression, thereby modulating epithelial-mesenchymal transition. Mediates beta-catenin dephosphorylation at adhesion junctions. Acts as a negative regulator of the oncogenic property of YAP, a downstream target of the hippo pathway, in a cell density-dependent manner. May function as a tumor suppressor.
Description:
PPTP-D2 (also known as Pez, PTP36 or PTPN14), Genbank accession number NM_005401, a.a. 817-1188 (catalytic domain), with N-terminal GST tag, MW=61 kDa, expressed in an E. coli expression system.
Synonym(s):
PTP-D2, PTPN14, Protein tyrosine phosphatase D2, Pez, PTP36
Specific Activity:
152 U/µg
Assay Conditions:
20 mM HEPES, pH 7.2, 100 mM NaCl, 2 mM EDTA, 1 mM DTT, 1 mM pNPP and PTPase. Assay was done at RT for 15 min, and the reaction was monitored at 405 nm continuously. Specific activity was calculated based on ε pNPP= 18,000 M-1cm-1.
Formulation:
25 mM Tris-HCl, pH 8.0, 75 mM NaCl, 0.05% Tween-20, 50% glycerol, 2 mM EDTA, 1 mM DTT, 10 mM glutathione.
Format:
Aqueous buffer solution
Storage / Stability:

>12 months at -80°C.

Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s):
1. Wadham, C. et al., J.Cell. Sci. 2000, 113: 3117-3123.
2. Ogata, M. et al. J. Biol.Chem. 1999, 274: 20717-20724.
Warning(s):
Avoid freeze/thaw cycles
Scientific Category:
PTP/Non-rec.
Product Type:
Recombinant Protein
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Product Datasheets

30025_Lot110607.pdf