Bas (PTPN13), GST-tag

Catalog #: 30024
Size: 20 µg
$375
Species: Human
Host Species: E. coli
MW: 71.8 kDa
Genbank #: NM_080685
Tag(s): N-terminal GST-tag
a.a: 2091–2490
Background:
PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. PTPN13 is a large protein that possesses a PTP domain at C-terminus, and multiple noncatalytic domains, which include a domain with similarity to band 4.1 superfamily of cytoskeletal-associated proteins, a region consisting of five PDZ domains, and a leucine zipper motif. PTPN13 binds to a negative regulatory domain in Fas that inhibits Fas-induced apoptosis.
Description:
Human, recombinant PTP-Bas, catalytic domain (Gene bank accession number NM_080685), a.a. 2091-2490 (catalytic domain), with N-terminal GST tag, MW=71.8 kDa, expressed in an E. coli expression system.
Synonym(s):
Bas, FAP-1, PTPN13, APO-1/CD95-Associated Phosphatase 1, PTP-BAS, PNP1, PTP1E, PTPL1, PTPLE, PTP-BL, Fas-Associated Phosphatase 1
Specific Activity:
5 U/µg
Unit Definition:
One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C.
Assay Conditions:
The specific activity of Bas was determined using pNPP. Assay conditions: 50 mM HEPES, pH 7.4, 2 mM EDTA, 3 mM DTT, 100 mM NaCl, 20 mM pNPP, and 20 ng/µl enzyme for 5 minutes at 30°C.
Formulation:
25 mM Tris-HCl, pH 8.0, 75 mM NaCl, 0.05% Tween-20, 50% glycerol, 2 mM EDTA, 1 mM DTT, 10 mM glutathione.
Format:
Aqueous buffer solution
Storage / Stability:

>12 months at -80°C.

Application(s):
Useful for the study of enzyme kinetics and regulation, to dephosphorylate target substrates and for screening inhibitors.
Reference(s):
1. W. Kimber, et al., Biochem.J. 2003, 376: 525-535.
2. N. Kachel, et al. J.Mol. Biol. 2003, 334: 143-155.
Warning(s):
Avoid freeze/thaw cycles
Scientific Category:
PTP/Non-rec.
Product Type:
Recombinant Protein
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Product Datasheets

30024_Lot2000.pdf