Bas (PTPN13), GST-tag Recombinant

Catalog #

30024

$325 *

Size: 50 µg

Qty

Bulk/Custom

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Description

Human, recombinant PTP-Bas, catalytic domain (Gene bank accession number NM_080685), a.a. 2091-2490 (catalytic domain), with N-terminal GST tag, MW=71.8 kDa, expressed in an E. coli expression system.

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Synonyms

Bas, FAP-1, PTPN13, APO-1/CD95-Associated Phosphatase 1, PTP-BAS, PNP1, PTP1E, PTPL1, PTPLE, PTP-BL, Fas-Associated Phosphatase 1

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

E. coli

Format

Aqueous buffer solution

Formulation

25 mM Tris-HCl, pH 8.0, 75 mM NaCl, 0.05% Tween-20, 50% glycerol, 2 mM EDTA, 1 mM DTT, 10 mM glutathione.

71.8 kDa

Amino Acids

2091–2490

Specific Activity

5 U/µg

Unit Definition

One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C.

Genbank #

NM_080685

UniProt #

Q12923

Tag(s)

N-terminal GST-tag

Background

PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. PTPN13 is a large protein that possesses a PTP domain at C-terminus, and multiple noncatalytic domains, which include a domain with similarity to band 4.1 superfamily of cytoskeletal-associated proteins, a region consisting of five PDZ domains, and a leucine zipper motif. PTPN13 binds to a negative regulatory domain in Fas that inhibits Fas-induced apoptosis.

References

1. W. Kimber, et al., Biochem.J. 2003, 376: 525-535.
2. N. Kachel, et al. J.Mol. Biol. 2003, 334: 143-155.

Storage/Stability

>12 months at -80°C.

Assay Conditions

The specific activity of Bas was determined using pNPP. Assay conditions: 50 mM HEPES, pH 7.4, 2 mM EDTA, 3 mM DTT, 100 mM NaCl, 20 mM pNPP, and 20 ng/µl enzyme for 5 minutes at 30°C.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics and regulation, to dephosphorylate target substrates and for screening inhibitors.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

PTP/Non-rec.

Regulatory Status

Research Use Only