PP2A-α, His-tag/PP2AR, GST-tag

Catalog #: 30056
Size: 20 µg
$390
Species: Human
Host Species: Sf9 insect cells
MW: 36 kDa (PP2A-α); 92 kDa (PP2AR)
Genbank #: NM_002715 (PP2A-α); NM_014225 (PP2AR)
Tag(s): N-terminal GST-tag
a.a: 2-309 (PP2A-α); 2-589 (PP2AR)
Description:
Protein complex of human protein phosphatase 2A catalytic subunit  (PP2A), also known as TPD3, RP-C, PP2Ac, and PPP2CA (GenBank Accession NM_002715, a.a. 2-309 with C-terminal Histag) and human protein phosphatase 2A regulatory subunit (PP2AR) (GenBank Accession NM_014225, a.a. 2-589 with Nterminal GST-tag). Co-expressed in a baculovirus-infected Sf9 cell expression system. MW = 36 kDa (PP2A) and 92 kDa (PP2AR).
UniProt
P67775; P30153
Synonym(s):
protein phosphatase 2A catalytic subunit α, TPD3, RP-C, PP2Ac,PPP2CA, protein phosphatase 2A regulatory subunit, PP2AR
Specific Activity:

≥100 pmol/min/μg

Assay Conditions:
Assay Conditions: 20 mM HEPES, pH 7.2, 100 mM NaCl, 3 mM DTT, 10 mM pNPP and PP2A. Assay was done at 37°C for 40 min, and the reaction progress was measured at 405 nm. Specific activity was calculated based on  pNPP= 18,000 M-1cm-1.
Formulation:
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 20% glycerol and 16 mM glutathione
Storage / Stability:

At least 6 months at –80°C.

Application(s):
Useful for the study of enzyme kinetics and for inhibitor screening.
Reference(s):
1. Van Hoof, C. et al., Genomics. 1995; 28: 261-272.
2. Chao, Y. et al., Molec. Cell. 2006; 23: 535-546.
Warning(s):
Avoid freeze/thaw cycles. Storing diluted protein is not recommended, if necessary, use carrier protein (BSA 0.1 – 0.5%).
Scientific Category:
PTP/Serine
Product Type:
Recombinant Protein
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Product Datasheets

30056_Lot150914-G.pdf