Mammalian Expression Services
Our Mammalian Expression System offers flexibility you need to express and produce the recombinant protein of your choice.
BPS will clone your insert into the desired mammalian expression vector. Either standard subcloning or PCR cloning will be used to transfer the gene of interest into the expression vector. Clones will then be screened for the correct restriction fragments and sequenced.
Optimization of Expression and Protein Characterization
The mammalian expression vector will be transiently transfected into suspension 293 cells grown in a defined, serum-free medium. Recombinant protein production will be optimized in a small-scale culture by doing a time course of expression. For each test, the recombinant protein will be analyzed by SDS-PAGE and/or Western blot. In addition, the yield per unit volume will be estimated. Using the results of these experiments, a strategy for producing and purifying the protein will be made.
Production of the recombinant mammalian-expressed protein can be scaled up to 1 liter. You will receive the cell pellets or conditioned media and a complete project report with the SDS-PAGE/Western blot analysis that documents the protein production.
BPS will purify your recombinant proteins using an affinity column. Large scale purification is also available.