Baculovirus Expression Services
Our Baculovirus Expression System ensures you will have the expression and production of the recombinant protein of your choice.
BPS will clone your insert into a baculovirus transfer vector. Either a standard subcloning or a PCR cloning will be used to transfer the gene of interest into the vector. Clones will be screened for the correct restriction fragments and sequenced, if necessary.
Recombinant Virus Production
Using linearized DNA from Autographa californica nuclear polyhedrosis virus (AcMNPV), BPS will cotransfect a baculovirus transfer vector into Sf9 cells. The low –titer recombinant viruses will be amplified 2-3 times to generate high-titer virus stocks.
Optimization of Expression
Recombinant protein expression will be optimized in one or more cell lines by doing a time-course study of infection, varying the multiplicity of infection (MOI). At each time point, the expressed protein will be analyzed by SDS-PAGE and/or Western blot. The results of these experiments will be used to determine the optimal conditions for protein production.
Recombinant protein from a small-scale culture will be characterized for cellular location, solubility, and accessibility of purification tags. In addition, the yield per unit volume will be estimated. Using the results of these experiments, a strategy for producing and purifying the protein will be made.
Production of the recombinant protein can be scaled up between one and several liter volumes. You will receive the cell pellets or conditioned media and a complete project report with the SDS-PAGE and/or Western blot analysis that document protein production.
BPS will purify your recombinant proteins using an affinity column. Large scale purification is also available.