PADI2, FLAG-Tag Recombinant

Catalog #

50152

$330 *

Size: 10 µg

Qty

Bulk/Custom

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Description

Human PADI2, also known as Peptidyl arginine deiminase, type II, (GenBank Accession No. NM_007365), 1-665(end), with C-terminal FLAG-tag, MW= 77 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

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Synonyms

PADI-2, Peptidyl arginine deiminase, type II, Pad

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations

Species

Human

Construct

PADI2 (1-665(end)-FLAG)

Host Species/Expression System

Sf9 insect cells

Purity

≥84%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 80 ng/µl FLAG peptide, 3 mMDTT, and 20% glycerol.

77 kDa

Amino Acids

1-665(end)

Specific Activity

≥19 pmole/min/µg.

Genbank #

NM_007365

UniProt #

Q9Y2J8

Tag(s)

C-terminal FLAG-tag

References

1. Dong S, et al. (2005.) J. Invest. Dermatol. 124(5):1026-33.
2. Wantanabe Y, et al. (2009). J Hum Genet. 54 (7): 430–2.

Storage/Stability

At least 6 months at –80°C.

Assay Conditions

Enzyme reaction was done in a buffer (50 mM HEPES, pH 7.4, 50 mM NaCl, 10 mM CaCl₂, and 2 mM DTT) containing PADI and 10 mM BAEE as a substrate for 30 min at 37°C. Amount of ammonia produced was measured and used for specific activity calculation.
After 30 min incubation at 37°C, the reaction was quenched using 15 µL of 500 mM EDTA (final concentration is 115 mM) followed by measuring the amount of NH3 produced by o-phthalaldehyde (OPT) and dithiothreitol (DTT). Fluorescence was measured at λ_ex413 nm and λ_em476 nm.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Arginine Deiminase

Regulatory Status

Research Use Only