Sortase Sampling Kit

Catalog #
79709
$675 *
Size: 50 µg
Qty
*US Pricing only. For international pricing, please contact your local distributor.
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Description

Sortases catalyze a transpeptidation reaction, where the enzyme cleaves the amide bond between the threonine and glycine of the sortase recognition sequence (LPXTG for S. aureus Sortase A), generating a thioacyl intermediate. Subsequently, this intermediate is resolved by the N-terminus of an oligoglycine nucleophile, creating a new peptide bond that links the substrate to the incoming nucleophile. Sortase variants are available that may exhibit enhanced activity with the substrate of interest. This kit provides wildtype Sortase and four different mutants to evaluate which sortase is best for the chosen application.

Assay Principle

Product Info
Storage and Usage
Citations
Materials Required But Not Supplied

Target protein (1 mg/mL, 10 μg per reaction)
Poly-glycine label (5 mM, 1 μl per reaction, <10 kDa)
37°C incubator
Microcentrifuge
Electrophoresis sample buffer (for SDS-PAGE) or 1 M EDTA solution (for mass spectroscopy)

Format

Assay Kit Components

References

1. Popp M. Methods Mol Biol. 2015; 1266:185-98.
2. Chen L., et al., 2016 Scientific Reports 6: 31899