Rat, recombinant, C-terminal HIS tag
Catalog #: 80201
Size: 100 µg
Description: Rat, Activated Renin
(GenBank Accession No. NM_012642),
amino acids 65 – 402 (end) with C-terminal
HIS tag, MW=38.5 kDa, expressed in a
HEK293 cell expression system.
The assay was performed following the renin
assay kit (Anaspec, #72140). Enzymatic
reactions were conducted at 37°C for 60
minutes in a 100 μl mixture containing
assay buffer, 2.5 μM 5-FAM/QXL™ 520
FRET Peptide, and rat Renin enzyme.
Fluorescence intensity was measured at
an excitation of 490 nm and an emission
of 520 nm using a Tecan Infinite M1000
Application: Useful for the study of enzyme
kinetics, screening inhibitors, and selectivity
Formulated in: 40 mM Tris-HCl, pH 8.0,
110 mM NaCl, 2.2 mM KCl, and 20%
Stability: >6 months at –80°C
1. Kedrah, AE., et al., Kidney Blood Press
Res. 2012 Jun 6;35(6):425-430.
2. Burnham, CE., et al., Proc Natl Acad
Sci USA. 1987 Aug;84(16):5605-5609.
PRODUCT DATA SHEETS
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