HCV1b (D168V), FLAG-His-tags

Catalog #: 80110
Size: 20 µg
Species: Human
Host Species: E. coli
MW: 22.3 kDa
Genbank #: AF054247
Tag(s): N-terminal FLAG-His-tags
a.a: 21–32 of NS4A, linker (3 a.a.), a.a. 31–181 of NS3
Hepatitis C virus (HCV) genotype 1b, also known as HCV1b, is a fusion protein of a.a. 21-32 of NS4A, linker (4 a.a.) and a.a. 3-181 of NS3 (GenBank Accession No. AF054247) with Asp-to-Val mutation on a.a. 168, and N-terminal FLAG-His-tag, MW = 22.3 kDa, expressed in an E. coli expression system.
hepatitis c virus genotype 1b V168
Specific Activity:
45 pmole/min/µg
Assay Conditions:
Assay was performed in 100 µl HCV assay buffer containing 50 mM Tris, pH7.4, 150 mM NaCl, 5 mM DTT, 10% glycerol, and 5 µM fluorescently labeled HCV substrate peptide. Reaction was continuously monitored at room temperature for 20 min. at ex350/em500.
40 mM Tris-HCl, pH 8.0, 400 mM NaCl, 0.2% Triton X-100, 10 mM ?-mercaptoethanol, 100 µg/ml FLAG peptide, and 30% glycerol
Aqueous buffer solution
Storage / Stability:

>6 months at -80°C.

1. Failla, C., et al., J. Virol. 1994 June;68(6):3753-3760.
2. Lang Kuhs, K.A., et al., Mol. Ther. 2012 May;20(3):669-678.
3. Massariol, MJ., et al., Biochem Biophys Res Commun. 2010 Jan 1;391(1):692-697.
Avoid freeze/thaw cycles
Scientific Category:
Product Type:
Recombinant Protein
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