FAP, His-tag

Catalog #: 80100
Size: 25 µg
$425
Species: Human
Host Species: Sf9 insect cells
MW: 85.5 kDa
Genbank #: NM_004460
Tag(s): C-terminal His-tag
a.a: 29–end
Background:
Fibroblast Activation Protein, or FAP, is a 170 kDa dimer with dipeptidase activity. FAP expression is seen on activated stromal fibroblasts in >90% of all human carcinomas, suggesting a role in the development, growth and metastasis of carcinomas. It is also involved in the control of wound healing and tissue repair.
Description:
Human FAP (GenBank Accession No. NM_004460), (a.a. 29-end) with C-terminal His-tag, MW=85.5 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
UniProt
Q12884
Synonym(s):
Fibroblast Activation Protein, seprase
Specific Activity:
≥ 83 pmol/min/µg.
Assay Conditions:
50 mM Tris-HCl (pH 7.5), 10 mM MgCl2, 1 mM MnCl2, 5 µM substrate (A-P-AMC), incubation 10 min at room temperature.
Formulation:
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 200 mM imidazole, and 20% glycerol.
Format:
Aqueous buffer solution
Storage / Stability:

>6 months at -80°C.

Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s):
1. Edosada,C.Y. et al., J. Biol. Chem. 281 (11), 7437-7444 (2006).
2. Edosada,C.Y. et al., FEBS Lett. 580 (6), 1581-1586 (2006).
Warning(s):
Avoid freeze/thaw cycles
Scientific Category:
Protease
Product Type:
Recombinant Protein
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Product Datasheets

80100_Lot131001-EB.pdf