DPP7, His-tag

Catalog #: 80070
Size: 10 µg
$415
Species: Human
Host Species: Sf9 insect cells
MW: 53 kDa
Genbank #: NM_013379
Tag(s): C-terminal His-tag
a.a: 26–end
Background:
Dipeptidyl peptidase-7 (DPP7), also known as DPP2, is a serine aminopeptidase found in quiescent lymphocytes. Inhibition of this enzyme induces apoptosis, and levels of DPP7 are elevated in many tumors, suggesting that DPP7 may be involved in a novel pathway for tumor suppression.
Description:
Human DPP7 (GenBank Accession No. NM_013379), a.a. 26-end with C-terminal His-tag, MW=52.5 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
UniProt
Q9UHL4
Synonym(s):
Dipeptidylpeptidase 7, QPP, Carboxytripeptidase, DPP2, DPP
Specific Activity:
≥ 1880 pmol/min/µg.
Assay Conditions:
Test wells contained 0.01M Tris-HCl pH7.4, 0.01M MgCl2, 0.05% Tween-20, 5 µM DPP substrate 1, and various amounts of DPP7(0-200ng). Standard wells contained 0.01M Tris-HCl pH7.4, 0.01M MgCl2, 0.05% Tween-20, and various amounts of Fluorescent AMC standard (0.01 µM-1.25 µM). Incubated for 10 minutes at room temperature. Fluorescence intensity was measure at excitation 360 nm and emission 460 nm.
Formulation:
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 220 mM imidazole, and 20% glycerol
Format:
Aqueous buffer solution
Storage / Stability:

>6 months at -80°C.

Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s):
1. Shreder K.R. et al., Bioorg Med Chem Lett. 2005 Oct 1;15(19):4256-60.
2. Maes M.B. et al., Biochem J. 2005 Mar 1;386(Pt 2):315-24.
Warning(s):
Avoid freeze/thaw cycles
Scientific Category:
Protease
Product Type:
Recombinant Protein
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Product Datasheets

80070_Lot_111207-2.pdf