DPP4, His-tag

Catalog #: 80040
Size: 10 µg
$420
Species: Human
Host Species: Sf9 insect cells
MW: 87 kDA
Genbank #: NM_001935
Tag(s): C-terminal His-tag
a.a: 29–end
Background:
Dipeptidyl peptidase-4 (DPP4 protein), also known as adenosine deaminase complexing protein 2, is a serine exopeptidase that cleaves X-proline dipeptides from the N-terminus of polypeptides. The DPP4 protein plays a key role in glucose metabolism, immune regulation, signal transduction and apoptosis.
Description:
Human DPP4 (GenBank Accession No. NM_001935), (amino acids 29-end) with C-terminal His tag, MW=87 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
UniProt
P27487
Synonym(s):
DPPIV, CD26, Dipeptidylpeptidase 4, ADCP2, DPP4 protein, DPP
Specific Activity:
8000 pmol/min/µg
Assay Conditions:
10 mM Tris-HCl, pH 7.4, 10mM MgCl2, 0.05% Tween 20, and 20μM DPP substrate 1. Incubate for 15 minutes at room temperature. Fluorescence intensity is measured at exc360/em460.
Formulation:
45 mM Tris-HCl pH 8.0, 124 mM NaCl, 2.4 mM KCl, 225 mM Imidazole and 10% glycerol
Format:
Aqueous buffer solution
Storage / Stability:

>6 months at -80°C.

Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s):

1. Deacon CF, Carr RD, and Holst JJ Front Biosci. 2008 Jan 1;13:1780-94.
2. Langley AK, Suffoletta TJ, and Jennings HRPharmacotherapy. 2007 Aug;27(8):1163-80.

Application Reference(s):
1. ZY15557, a novel, long acting inhibitor of dipeptidyl peptidase-4, for the treatment of Type 2 diabetes mellitus (2017)
2.The activity of circulating dipeptidyl peptidase-4 is associated with subclinical left ventricular dysfunction in patients with type 2 diabetes mellitus (2013)

Warning(s):
Avoid freeze/thaw cycles
Scientific Category:
Protease
Product Type:
Recombinant Protein
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Product Datasheets

80040_lot_170710-1.pdf