Cathepsin V, His-tag

Catalog #: 80009
Size: 10 µg
$375
Species: Human
Host Species: FreeStyle 293-F cells
MW: 37 kDa
Genbank #: NM_060911
Tag(s): C-terminal His-tag
a.a: CD33 1–16, CathepsinV 18–334
Description:
Human cathepsin V (GenBank Accession No. NM_060911), CD33 signal peptide(a.a. 1-16) + cathepsin V (a.a. 18-334), with C-terminal His-tag, MW=37 kDa, expressed in FreeStyle 293-F cells.
UniProt
O60911
Synonym(s):
CTSV
Specific Activity:

≥ 250 pmol/min/µg

Assay Conditions:
Assay is conducted in 25 mM MES buffer pH5.0, 0.1 M NaCl, 5 mM DTT and 20 μM substrate (Z-LeuArg-AMC) at room temperature for 30 minutes. Fluorescence intensity is measured at exc360/em460.
Formulation:
40 mM MES pH 5.0, 120 mM NaCl and 20% glycerol.
Format:
Aqueous buffer solution
Storage / Stability:

>6 months at -80°C.

Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s):
1. Puzer L. et al., Biochem. Biol. Chem. 389 (2), 195-200 (2008).
2. Burden R.E. et al., Biol. Chem. 388 (5), 541- 545 (2007).
Warning(s):
Avoid freeze/thaw cycles
Scientific Category:
Protease
Product Type:
Recombinant Protein
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Product Datasheets

80009 lot 160823.pdf