Cathepsin S, His-tag

Catalog #: 80008
Size: 10 µg
$365
Species: Human
Host Species: FreeStyle 293-F cells
MW: 37 kDa
Genbank #: NM_004079.3
Tag(s): C-terminal His-tag
a.a: CD33 1–16, CathepsinS 17–331
Description:
Human cathepsin S (GenBank Accession No. NM_004079.3), CD33 signal peptide(a.a. 1-16) + cathepsin S (a.a. 17-331), with C-terminal His-tag, MW=37 kDa, expressed in FreeStyle 293-F cells.
UniProt
P25774
Synonym(s):
CTSS
Specific Activity:
≥ 700 pmole/min/µg
Assay Conditions:
The enzymes were diluted in 25 mM MES buffer pH5, 0.1 M NaCl, 5 mM DTT, and activated by low pH at room temperature for 2 hours. After addition of 10 μM substrate (Z-L-R-AMC) a kinetic reaction was followed for 30 minutes at room temperature and the fluorescence intensity was measured at exc360/em460.
Formulation:
45 mM Tris7HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 225 mM imidazole, 3 mM DTT, and 10% glycerol
Format:
Aqueous buffer solution
Storage / Stability:

>6 months at -80°C.

Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s):
1. Seo H.R. et al., Int. J. Cancer 124 (8), 1794-1801 (2009).
2. Lutzner N. and Kalbacher H. J. Biol. Chem. 283 (52), 36185-36194 (2008).
Warning(s):
Avoid freeze/thaw cycles
Scientific Category:
Protease
Product Type:
Recombinant Protein
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Product Datasheets

80008_Lot 170214.pdf