Cathepsin L, His-tag

Catalog #: 80005
Size: 10 µg
$365
Species: Human
Host Species: FreeStyle 293-F cells
MW: 37 kDa
Genbank #: NM_001912
Tag(s): C-terminal His-tag
a.a: 18–333
Description:
Human cathepsin L (GenBank Accession No. NM_001912), a.a. 18-333, with C-terminal His-tag, MW=37 kDa, expressed in FreeStyle 293-F cells.
UniProt
P07711
Synonym(s):
CTSL, MEP
Specific Activity:
≥ 6000 pmol/min/µg
Assay Conditions:
The enzyme reactions were conducted in 50mM MES buffer pH5.0, 100mM NaCl, 5mM DTT and 20μM substrate (Z-Leu-Arg-AMC) 30 minutes at room temperature. The fluorescence intensity was measured at exc360/em460.
Formulation:
40 mM Tris-HCl pH 8.0, 110 mM NaCl, 2.2 mM KCl, 250 mM Imidazole, and 20% glycerol
Format:
Aqueous buffer solution
Storage / Stability:

>6 months at -80°C.

Application(s):
Useful for activation of DPP1 (Cathepsin C). Suitable for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s):
1. Jean D. et al., Biochem. J. 413 (1), 125-134 (2008).
2. Abboud-Jarrous G. et al., J. Biol. Chem. 283 (26), 18167-18176 (2008).
Warning(s):
Avoid freeze/thaw cycles
Scientific Category:
Protease
Product Type:
Recombinant Protein
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Product Datasheets

80005_Lot_170620-1.pdf