Cathepsin F, His-tag

Catalog #: 80003
Size: 50 µg
$365
Species: Human
Host Species: FreeStyle 293-F cells
MW: 53 kDa
Genbank #: NM_003793
Tag(s): C-terminal His-tag
a.a: CD33 1–16, CathepsinF 20–end
Description:
Human cathepsin F (GenBank Accession No. NM_003793), CD33 signal peptide(a.a. 1-16) + cathepsin F (a.a. 20-end), with C-terminal His-tag, MW = 53 kDa (more than 58kDa after post-modification), expressed in FreeStyle 293-F cells.
UniProt
Q9UBX1
Synonym(s):
CTSF, CATSF
Specific Activity:
≥ 0.9 pmole/min/µg.
Assay Conditions:
Enzyme activity was measured in a buffer containing 100 mM Sodium acetate (pH 5.5), 2 mM EDTA, 8 mM DTT, 0.05% Brij, and 20 µM substrate (Z-FRAMC), with 3.75-30 ng/µl Cathepsin F. Reaction was done at 30°C for 40-120 min, and fluorescence signal was measured at Ex360 nm/Em460 nm. Enzyme was preactivated in 50 mM sodium acetate pH 4.5, 5 mM DTT buffer for 90 min at RT prior to reaction.
Formulation:
45 mM sodium acetate, 135 mM NaCl and 10% glycerol
Format:
Aqueous buffer solution
Storage / Stability:

>6 months at -80°C.

Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s):
Warning(s):
Avoid freeze/thaw cycles
Scientific Category:
Protease
Product Type:
Recombinant Protein
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Product Datasheets

80003 lot 160112B.pdf