Caspase9, His-tag

Catalog #: 80115
Size: 50 µg
$355
Species: Human
Host Species: E. coli
MW: see Description
Genbank #: NM_001229
Tag(s): C-terminal His-tag
a.a: 1 – 416 (end)
Description:
Human Caspase 9 (CASP9), GenBank Accession No. NM_001229, a.a. 1 - 416 (end), with C-terminal His-tag, MW=35 &10 kDa, expressed in an E. coli cell expression system. Procaspase-9 is autocleaved between the prodomain and large protease subunit (p35). The cleavage product contains both the large (p35) and small (p10 [His-tag]) protease subunits.
UniProt
P55211
Synonym(s):
Caspase-9, CASP9, Caspase 9, Mch6, ICE-LAP6
Specific Activity:
≥ 0.11 pmol/min/µg
Assay Conditions:
50 mM HEPES, pH 7.2, 50 mM NaCl, 10 mM EDTA, 0.1% Chaps, 5% glycerol, 10 mM DTT, and 10 µM Caspase 9 substrate. Incubate for 30 minutes at room temperature. Fluorescence intensity is measured at exc380/em505 nm.
Formulation:
50 mM Tris pH 8, 110 mM NaCl, 2.2 mM KCl, 20% glycerol, 3 mM DTT, 80 mM imidazole.
Format:
Aqueous buffer solution
Storage / Stability:
>6 months at -80°C.
Application(s):
Useful for the study of enzyme kinetics, screening inhibitors,and selectivity profiling.
Reference(s):
1. Guerrero AD., et al., J Clin Cell Immunol.9;3(3) (2012)
2. Luo W., et al., Int J Biol Macromol. S0141-8130(13)00222-5. (2013)
Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
Protease
Product Type:
Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.

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80115_Lot130426E100.pdf