Caspase8, His-tag

Catalog #: 80114
Size: 50 µg
$435
Species: Human
Host Species: E. coli
MW: see Description
Genbank #: NM_001228
Tag(s): C-terminal His-tag
a.a: 200 – 496 (end)
Background:
Caspase-8 is a member of the caspase (cysteine aspartate protease) family of proteins, and has been shown to be an executioner protein of apoptosis. Caspase-8 has been associated with chronic inflammation and neurodegenerative disorders including Alzheimer’s, Huntington’s, and Parkinson’s.
Description:
Human Caspase 8 (CASP8), GenBank Accession No. NM_001228), a.a. 200 - 496 (end), with C-terminal His-tag, MW=34.5 kDa, expressed in an E. coli cell expression system. Procaspase-8 is autocleaved between the prodomain and large protease subunit (p18). The cleavage product, p30, contains both the large (p18) and small (p10 [His-tag]) protease subunits. p30 is further processed to the p18 and p10 subunits, which dimerize to form active enzyme.
UniProt
Q14790
Synonym(s):
Caspase-8, CASP8, Caspase 8, FLICE, MACH, Mch5
Specific Activity:
≥ 1145 pmol/min/µg
Assay Conditions:
Assayed using BPS's Homogeneous Caspase-8 Assay Kit, Cat. # 80704. 50 mM HEPES, pH 7.2, 50 mM NaCl, 10 mM EDTA, 0.1% Chaps, 5% glycerol, 10 mM DTT, and 20 µM Caspase 8 substrate. Incubate for 30 minutes at room temperature. Fluorescence intensity is measured at exc380/em505 nm.
Formulation:
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 200 mM imidazole, 3 mM DTT and 20% glycerol.
Format:
Aqueous buffer solution
Storage / Stability:
>6 months at -80°C.
Application(s):
Useful for the study of enzyme kinetics, screening inhibitors,and selectivity profiling.
Reference(s):
1. MacPherson, G., et al., J. Natl.Cancer Inst. 2004 Dec 15;96(24):1866-9.
2. Sun, T., et al., Nat Genet. 2007 May;39(5):605-13.
3. Hoffman, J.C., et al. Mol. Cell. Biol. 2009 Aug; 29(16): 4431-4440.
Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
Protease
Product Type:
Recombinant Protein
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80114_Lot130215-2.pdf