Caspase7, His-tag

Catalog #: 70000
Size: 50 µg
$435
Species: Human
Host Species: E. coli
MW: 35.1 kDa
Genbank #: NM_001227
Tag(s): C-terminal His-tag
a.a: full length
Background:
Caspase-7 is a member of the caspase (cysteine aspartate protease) family of proteins, and has been shown to be an executioner protein of apoptosis.
Description:
Human Caspase-7 (full length) (GenBank Accession No. NM_001227) with C-terminal His-tag, MW= 35.1 kDa, expressed in an E. coli expression system. Active caspase 7 is a heterotetramer consisting of two copies each of ~20 kDa and ~11 kDa subunits.
UniProt
P55210
Synonym(s):
Caspase-7, CASP7, Caspase 7, Mch3, ICE-LAP3
Specific Activity:
≥ 5337 pmol/min/µg
Assay Conditions:
Assayed using BPS's Homogeneous Caspase-7 Assay Kit, Cat. # 80701. 10 mM HEPES, pH 7.4, 10 mM EDTA, 0.05% Chaps, 5 mM DTT, and 10 µM Caspase substrate. Incubate for 30 min at RT. Fluorescence intensity measured at exc380/em500 nm
Formulation:
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 155 mM imidazole, 20% glycerol and 3 mM DTT.
Format:
Aqueous buffer solution
Storage / Stability:
>6 months at -80°C.
Application(s):
Useful for the study of enzyme kinetics, screening inhibitors,and selectivity profiling.
Reference(s):
Wang DH., et al., PLoS one,7(6):e39920. doi:10.1371/journal.0039920. (2012).
Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
Protease
Product Type:
Recombinant Protein
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Product Datasheets

70000_Lot130116.pdf