Caspase6, His-tag

Catalog #: 80113-1
Size: 10 µg
$250
Catalog #: 80113-2
Size: 50 µg
$435
Species: Human
Host Species: E. coli
MW: See Description
Genbank #: NM_001226
Tag(s): C-terminal His-tag
a.a: 1–293 (end)
Background:
Caspase-6 is a member of the caspase (cysteine aspartate protease) family of proteins, and has been shown to be an executioner protein of apoptosis. Recent evidence shows that caspase-6 is involved in neurodegenerative diseases, such as Huntington’s and Alzheimer’s.
Description:
Human Caspase 6 (CASP6), GenBank Accession No. NM_001226, full length, a.a. 1-293 (end), with C-terminal His-tag, expressed in an E. coli cell expression system. Pro-caspase-6 is activated by autocleavage to yield 20 kDa and 18 kDa proteins (large subunit) and an 11 kDa small subunit (His-tag) that dimerize to form the active enzyme.
UniProt
P55212
Synonym(s):
Caspase-6, CASP6, Caspase 6, Mch2
Specific Activity:
≥ 1720 pmol/min/µg
Assay Conditions:
Assayed using BPS's Homogeneous Caspase-6 Assay Kit, Cat. # 80703. 50 mM HEPES, pH 7.2, 50 mM NaCl, 10 mM EDTA, 0.1% Chaps, 5% glycerol, 10 mM DTT, and 20 µM Caspase 6 substrate. Incubate for 30 minutes at room temperature. Fluorescence intensity is measured at exc380/em505 nm.
Formulation:
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 320 mM imidazole, 0.01% Tween-20, and 20% glycerol.
Format:
Aqueous buffer solution
Storage / Stability:
>6 months at -80°C.
Application(s):
Useful for the study of enzyme kinetics, screening inhibitors,and selectivity profiling.
Reference(s):
1. Halawani, D., et al., J Neurosci. 2010 Apr 28;30(17):6132-42.
2. LeBlanc, A., et al., J Biol Chem. 1999 Aug 13;274(33):23426-36.
Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
Protease
Product Type:
Protein
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Product Datasheets

80113_Lot130222-2.pdf