Cathepsin S Inhibitor Screening Assay Kit
The Cathepsin S Inhibitor Screening Assay Kit is designed to measure the protease activity of Cathepsin S for screening and profiling applications. The Cathepsin S assay kit comes in a convenient 96-well or 384-well format, with enough purified Cathepsin S (amino acids 17-331), its substrate, and Cathepsin Buffer for 96 or 384 reactions. This kit includes the inhibitor E-64 as control.
Figure 1: Illustration of the assay principle.
The substrate is an internally quenched fluorogenic substrate. Proteolysis releases the highly fluorescent Mca from the quencher. Fluorescence intensity increases proportionally to the activity of the protease.
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- Adjustable micropipettor and sterile tips
- Fluorescence plate reader capable of measurement at λex360/λem460 nm.
96 reactions
Catalog # | Name | Amount | Storage |
80008 | Cathepsin S, His-Tag* | 4 µg | -80°C |
80349 | Fluorogenic Cathepsin Substrate 1 (5 mM) | 10 µl | -20°C |
4x Cathepsin Buffer | 2 ml | -20°C | |
0.5 M DTT | 200 µl | -20°C | |
1 mM E-64 | 10 µl | -20°C | |
79685 | 96-well black microplate | 1 | Room Temp |
* The concentration of protein is lot-specific and will be indicated on the tube containing the protein.
384 reactions
Catalog # | Name | Amount | Storage |
80008 | Cathepsin S, His-Tag* | 4 µg | -80°C |
80349 | Fluorogenic Cathepsin Substrate 1 (5 mM) | 2 x 10 µl | -20°C |
4x Cathepsin Buffer | 2 x 2 ml | -20°C | |
0.5 M DTT | 2 x 200 µl | -20°C | |
1 mM E-64 | 10 µl | -20°C | |
79961 | 384-well black microplate | 1 | Room Temp |
* The concentration of protein is lot-specific and will be indicated on the tube containing the protein.
Cathepsin S is a cysteine protease of the C1 papain family, found inside the lysosomal/endosomal compartments of antigen-presenting cells such as B cells, macrophages, and dendritic cells. Cathepsin S participates in the degradation of antigenic proteins to peptides for presentation. Contrary to other cathepsins, Cathepsin S functions at neutral pH and it is secreted in response to inflammatory triggers. Its activity is regulated by cystacin C, its endogenous inhibitor. Cathepsin S has additional biological effects, acting as one of the most potent elastases, cleaving a number of extracellular matrix (ECM) proteins. Its role as elastase contributes to blood vessel permeability and angiogenesis. High levels of this protein can be found in psoriatic keratinocytes and pulmonary fibrosis, and it has also been linked to nociception. The development of inhibitors targeting Cathepsin S can prove beneficial in the treatment of pulmonary fibrosis and other Cathepsin S-linked diseases.
Yoo Y., et al., 2022 Biomedicine & Pharmacotherapy 145: 112245.