The Renin Assay Kit is designed to measure Renin activity for screening and profiling applications. It comes in a convenient 96-well format, with purified, activated renin, renin substrate, and assay buffer for 100 enzyme reactions. Aliskiren Hydrochloride is also included as a control for renin inhibition. The key to the Renin Assay Kit is the specific, fluorogenic substrate. Using this kit, only one simple step on a microtiter plate is required for renin reactions. The fluorometric substrate is incubated with a sample containing renin enzyme to produce a fluorophore that can then be measured using a fluorescence reader.
Need us to run inhibitor screens or profile your compounds against Renin? Check out our Protease Screening Services.
1. Paschalidou, K. et al., Biochem. J. 2004; 382: 1031. 2. Gossas, T., et al., Naunyn Schmiedebergs Arch Pharmacol. 2012; 385(2):219-24.
Storage/Stability
6 months from date of receipt when stored as directed.
Instructions for Use
See assay kit data sheet for detailed protocol.
Applications
Great for studying enzyme kinetics, screening small molecular inhibitors for drug discovery and HTS applications.
Shipping Temperature
-80°C
Contraindications
DMSO >1%, strong acids or bases, ionic detergents, high salt
Warnings
Avoid freeze/thaw cycles
Disclaimers and Limitations
BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.
End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.