Renin Assay Kit

Catalog #
80211
$450 *
Size: 96 reactions
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Description

The Renin Assay Kit is designed to measure Renin activity for screening and profiling applications. It comes in a convenient 96-well format, with purified, activated renin, renin substrate, and assay buffer for 100 enzyme reactions. Aliskiren Hydrochloride is also included as a control for renin inhibition. The key to the Renin Assay Kit is the specific, fluorogenic substrate. Using this kit, only one simple step on a microtiter plate is required for renin reactions. The fluorometric substrate is incubated with a sample containing renin enzyme to produce a fluorophore that can then be measured using a fluorescence reader.

Need us to run inhibitor screens or profile your compounds against Renin? Check out our Protease Screening Services.

This product has been cited 3 times.

Synonyms
Angiotensinogenase, REN
Product Info
Storage and Usage
Citations3
Assay Kit Format
Fluorogenic
Species
Human
Supplied As
Supplied in a convenient 96-well format, with purified, activated renin, renin substrate, assay buffer, and inhibitor for 100 enzyme reactions.
Materials Required But Not Supplied

Fluorescent microplate reader

Format
Catalog # Component Amount Storage
80200 Activated Renin 2 µg -80°C Avoid freeze/ thaw cycles!
  PR-02 Assay Buffer 25 ml -20°C
  Renin Substrate 30 µl @ 1 mM -80°C
27300 Aliskiren Hydrochloride 20 µl @ 100 μM in DMSO -20°C
79685 Black 96-well plate   RT
UniProt #
P00797
References

1. Paschalidou, K. et al., Biochem. J. 2004; 382: 1031.
2. Gossas, T., et al., Naunyn Schmiedebergs Arch Pharmacol. 2012; 385(2):219-24.