Caspase-8 Homogeneous Assay Kit

Catalog #

80704

$385 *

Size: 96 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The Homogeneous Caspase-8 Assay Kit is a complete assay system designed to measure Caspase-8 activity for screening and profiling applications. It comes in a convenient 96-well format, with all the reagents necessary for 100 fluorescent Caspase-8 activity measurements. In addition, the kit includes purified Caspase-8 enzyme and a potent Caspase-8 inhibitor, Ac-IETD-CHO, for use as a positive and negative control. Using this kit, only one simple step on a microtiter plate is needed to analyze the Caspase-8 activity level. The fluorogenic substrate, Ac-IETD-AFC, is incubated with purified Caspase-8 and the enzymatic activity releases AFC fluorophore that can then be measured using a fluorescence reader.

Need us to run inhibitor screens or profile your compounds against Caspase-8? Check out our Apoptosis Screening Services or Protease Screening Services.

This product has been cited 3 times.

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Synonyms

CASP8, Caspase 8, FLICE, MACH, Mch5, Caspase

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Product Data Gallery

Product Info

Storage and Usage

Citations3

Assay Kit Format

Fluorogenic

Format

UniProt #

Q14790

Storage/Stability

6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Instructions for Use

See assay kit data sheet for detailed protocol.

Applications

Useful for studying enzyme kinetics and HTS applications.

Shipping Temperature

-80°C

Contraindications

DMSO >1%, strong acids or bases, ionic detergents, high salt

Warnings

Avoid freeze/thaw cycles.

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Protease

Regulatory Status

Research Use Only

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Caspase-8 Homogeneous Assay Kit

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