Caspase-3 Homogeneous Assay Kit

Catalog #: 80700
Size: 96 reactions
$275
Background:
Caspase-3 is a member of the caspase (cysteine aspartate protease) family of proteins, and has been shown to be an executioner protein of apoptosis.
Description:
The Homogeneous Caspase-3 Assay Kit is a complete assay system designed to measure Caspase-3 activity for screening and profiling applications. It comes in a convenient 96-well format, with all the reagents necessary for 100 fluorescent Caspase-3 activity measurements. In addition, the kit includes purified Caspase-3 enzyme and a potent Caspase-3 inhibitor, Ac-DNLD-CHO, for use as a positive and negative control. Using this kit, only one simple step on a microtiter plate is needed to analyze the Caspase-3 activity level. The fluorogenic substrate, Ac-DEVD-AFC, is incubated with purified Caspase-3 and the enzymatic activity releases AFC fluorophore that can then be measured using a fluorescence reader.
Synonym(s):
CASP3, caspase 3, CPP32, apopain, Yama, AlphaLISA, Alphascreen, Alpha Lisa, Alpha screen
Contraindications:
DMSO >1%, strong acids or bases, ionic detergents, high salt
Format:
Instructions for use:
See assay kit data sheet for detailed protocol.
Storage / Stability:

12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Application(s):
Great for studying enzyme kinetics and HTS applications.
Reference(s):
1. Liu, J., et al., Bioorg. Med. Chem. Lett., 1999 Nov 15;9(22):3231-6.
2. Riedl, S.J., et al., Cell, 2001 Mar 9;104(5):791-800
Notes:
AlphaScreen® and AlphaLISA® are registered trademarks of PerkinElmer, Inc.
Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
Protease
Product Type:
Assay Kit
Data shown is lot-specific. Contact us for specific information on other lots.

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Product Datasheets

80700.pdf