DPP9 Assay Kit

Catalog #

80209

$615 *

Size: 96 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

Dipeptidyl peptidase-9 (DPP9) is a serine exopeptidase that cleaves X-proline dipeptides from the N-terminus of polypeptides. DPP9 exhibits similar activity to that of DPP4 but unlike DPP4, DPP9 is not membrane bound. DPP9 activity has been linked to several diseases, including type-2 diabetes, obesity and cancer. The Fluorogenic DPP9 Assay Kit is designed to measure DPP9 activity using purified DPP9 for screening and profiling applications. Using this kit, only one simple step on a microtiter plate is required for DPP9 reactions. The fluorogenic substrate is incubated with a sample containing DPP9 enzyme to produce a fluorophore that can then be measured using a fluorescence reader.

Need us to run inhibitor screens or profile your compounds against DPP9? Check out our Protease Screening Services.

This product has been cited 6 times.

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Synonyms

Dipeptidylpeptidase 9, DPPIX, DPP

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Product Data Gallery

Product Info

Storage and Usage

Citations6

Assay Kit Format

Fluorogenic

Supplied As

Supplied in a convenient 96-well format, with purified DPP9 enzyme, DPP substrate, and DPP assay buffer for 100 enzyme reactions.

Format

Catalog Number	Component	Amount	Storage
80090	DPP9 human recombinant enzyme	10 µg	-80°C	Avoid freeze/ thaw cycles!
80300	DPP assay buffer	10 ml	-20°C
80305	Fluorogenic DPP substrate 1 in DMSO (0.5 mM)	100 µl	-80°C
	AMC Fluorescent standard (50 µM)	500 µl	-20°C
79685	black, low binding NUNC black microtiter plate	1 plate	Room temp.

UniProt #

Q86TI2

References

1. Bjelke,J.R. et al., Biochem. J. 396 (2), 391- 399 (2006).
2. Yu, D.M. et al., FEBS J. 273 (11), 2447-2460 (2006).

Storage/Stability

At least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Instructions for Use

See assay kit data sheet for detailed protocol.

Applications

Useful for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.

Shipping Temperature

-80°C

Notes

The AMC standard is included so the researcher can quatitatively determine the specific activity of the enzyme using the AMC standard as a measure of how much AMC substrate was cleaved to release free AMC.

Contraindications

DMSO >1%, strong acids or bases, ionic detergents, high salt

Warnings

Avoid freeze/thaw cycles

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Protease

Regulatory Status

Research Use Only