Cathepsin L Inhibitor Screening Assay Kit
The Cathepsin L Inhibitor Screening Assay Kit is designed to measure the protease activity of Cathepsin L for screening and profiling applications. The Cathepsin L assay kit comes in a convenient 96-well or 384-well format, with purified Cathepsin L (amino acids 18-333), its substrate, and Cathepsin Buffer for 96 or 384 reactions. This kit includes the inhibitor E-64 as control.
Figure 1: Illustration of the assay principle.
The substrate is an internally quenched fluorogenic substrate. Proteolysis releases the highly fluorescent substrate from the quencher. Fluorescence intensity increases proportionally to the activity of the protease.
Need us to run inhibitor screens or profile your compounds against Cathepsin L? Check out our Protease Screening Services or Coronavirus Services.
This product has been cited 12 times.
- Adjustable micropipettor and sterile tips
- Fluorescence plate reader capable of measurement at λex360/λem460 nm.
96 reactions
| Catalog # | Name | Amount | Storage |
| 80005 | Cathepsin L, His-Tag* | ˃1 µg | -80°C |
| 80349 | Fluorogenic Cathepsin Substrate 1 (5 mM) | 10 µl | -20°C |
| 4x Cathepsin Buffer | 2 ml | -20°C | |
| 0.5 M DTT | 200 µl | -20°C | |
| 1 mM E-64 | 10 µl | -20°C | |
| 79685 | 96-well black microplate | 1 | Room Temp |
* The concentration of protein is lot-specific and will be indicated on the tube containing the protein.
384 reactions
| Catalog # | Name | Amount | Storage |
| 80005 | Cathepsin L, His-Tag* | 1 µg | -80°C |
| 80349 | Fluorogenic Cathepsin Substrate 1 (5 mM) | 2 x 10 µl | -20°C |
| 4x Cathepsin Buffer | 2 x 2 ml | -20°C | |
| 0.5 M DTT | 2 x 200 µl | -20°C | |
| 1 mM E-64 | 10 µl | -20°C | |
| 79961 | 384-well black microplate | 1 | Room Temp |
* The concentration of protein is lot-specific and will be indicated on the tube containing the protein.
Cathepsin L is a lysosomal cysteine protease that belongs to the papain-like superfamily. It is involved in many essential physiological processes, including degradation and renewal of intracellular proteins, activation of prohormones, presentation of antigens, as well as organ development. One of its main functions is in the degradation of antigens, and it has been shown to participate in SARS-CoV-1 and SARS-CoV-2 infection, as infection requires viral membrane fusion and that seems to require proteolysis of the Spike protein by Cathepsin L. The use of amantadine inhibited Cathepsin L and prevented SARS-CoV-2 infection. Cathepsin L plays important roles in tumor metastasis and chemotherapy resistance, and upregulation of this enzyme has been reported in a wide range of human cancers including ovarian, renal, and breast carcinoma. The development of inhibitors targeting this protein may prove beneficial for both COVID-19 and cancer therapy.
Zhao M., et al., 2021 Signal Transduction and Targeted Therapy 6:134.
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