Cathepsin B Inhibitor Screening Assay Kit
The Cathepsin B Inhibitor Screening Assay Kit is designed to measure the protease activity of Cathepsin B for screening and profiling applications. The Cathepsin B assay kit comes in a convenient 96-well or 384-well format, with enough purified Cathepsin B (amino acids 18-339), its substrate, and Cathepsin Buffer for 96 or 384 reactions. This kit includes the inhibitor E-64 as control.
Figure 1: Illustration of the assay principle.
The substrate is an internally quenched fluorogenic substrate. Proteolysis releases the highly fluorescent Mca from the quencher. Fluorescence intensity increases proportionally to the activity of the protease.
Need us to run inhibitor screens or profile your compounds against Cathepsin B? Check out our Protease Screening Services.
This product has been cited 4 times.
- Adjustable micropipettor and sterile tips
- Fluorescence plate reader capable of measurement at λex330/λem390 nm.
96 reactions
| Catalog # | Name | Amount | Storage |
| 80001 | Cathepsin B, His-Tag* | 1 µg | -80°C |
| 80349 | Fluorogenic Cathepsin Substrate 1 (5 mM) | 10 µl | -20°C |
| 4x Cathepsin Buffer | 2 ml | -20°C | |
| 0.5 M DTT | 200 µl | -20°C | |
| 1 mM E-64 | 10 µl | -20°C | |
| 79685 | 96-well black microplate | 1 | Room Temp |
* The concentration of protein is lot-specific and will be indicated on the tube containing the protein.
384 reactions
| Catalog # | Name | Amount | Storage |
| 80001 | Cathepsin B, His-Tag* | 1 µg | -80°C |
| 80349 | Fluorogenic Cathepsin Substrate 1 (5 mM) | 2 x 10 µl | -20°C |
| 4x Cathepsin Buffer | 2 x 2 ml | -20°C | |
| 0.5 M DTT | 2 x 200 µl | -20°C | |
| 1 mM E-64 | 10 µl | -20°C | |
| 79961 | 384-well black microplate | 1 | Room Temp |
* The concentration of protein is lot-specific and will be indicated on the tube containing the protein.
Cathepsin B is a cysteine protease, of the C1 family of papain-like peptidases, that primarily functions as an endopeptidase within endo-lysosomal compartments in normal cells. Cathepsin B is closely linked to apoptosis, with activated caspase-8 leading to the release of cathepsin B to the cytosol, which in turn leads to cytochrome c release and apoptosis. It is also involved in Bcl-2 (B-cell lymphoma 2) degradation. It plays a role in inflammasome regulation, with NLRP3 (NOD and pyrin containing protein 3) activators increasing the interaction cathepsin-NLRP3 and caspase-1 activation. Cathepsin B-mediated programmed cell death (PCD) can be a cause of disease progression in abnormal tissues. High levels of cathepsin B are found in a wide variety of human cancers and in experimental models, such as transgenic models of murine pancreatic and mammary carcinomas and causal roles for this protein have been demonstrated in initiation, growth/tumor cell proliferation, angiogenesis, invasion, and metastasis. It has been proposed as a biomarker, and its levels seem to correlate with metastasis. Its multiple roles make cathepsin B an attractive target for cancer therapy.
Xie Z., et al., 2023 Cell Death & Disease 14: 255.
Chevriaux A., et al., 2020 Front. Cell Dev. Biol. 8: https://doi.org/10.3389/fcell.2020.00167.
Powered by Bioz
San Diego, CA 92121
858-202-1401
Products are for Research Use Only
COMPANY
Copyright © 2024
BPS Bioscience, Inc.
All rights reserved.
Diagrams on this website are created with BioRender.com.