BACE1 Assay Kit

Catalog #

71656

$530 *

Size: 96 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The BACE1 Assay Kit is designed to measure BACE1 activity for screening and profiling applications using a labeled peptide substrate in a homogeneous fluorogenic assay.

The peptide substrate contains a fluorescence Donor that is quenched by an Acceptor when the peptide is intact. Upon BACE1-mediated cleavage the two peptide fragments pull apart, releasing the highly fluorescent peptide fragment from quenching. Therefore, the fluorescence intensity increases proportionally to the activity of BACE1.

BACE1 FRET Assay Kit

Need us to run inhibitor screens or profile your compounds against BACE1? Check out our Protease Screening Services.

This product has been cited 1 time.

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Synonyms

beta-site amyloid precursor protein cleaving enzyme 1, ASP2

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Product Info

Storage and Usage

Citations1

Assay Kit Format

Fluorogenic

Supplied As

This Assay Kit comes in a convenient 96-well format, with enough purified recombinant human BACE1 enzyme, BACE1 peptide substrate, and assay buffer for 100 enzyme reactions.

Materials Required But Not Supplied

Microplate reader capable of reading fluorescence
Adjustable micropipettor and sterile tips

Format

Catalog Number	Reagent	Amount	Storage
71657	BACE1	20 µg	-80°C
	BACE1 assay buffer	12 ml	4°C
	BACE1 peptide substrate	100 µl	-80°C
79685	96-well plate, black	1	RT

UniProt #

P56817

Background

Bace1 (β-secretase 1, also known as beta-site amyloid precursor protein cleaving enzyme 1 and ASP2, GenBank Accession No. NM_012104.4) is an aspartic protease involved in the processing of the Amyloid precursor protein (APP). Cleavage of APP by BACE1 followed by γ-secretase results in β-amyloid peptide production, which ultimately leads to toxic Amyloid β accumulation. Amyloid β aggregation may play a critical role in Alzheimer’s disease pathogenesis, suggesting that BACE1 could be a potential therapeutic target in this disease.

References

Ghosh, A.K., et al. J. Neurochemistry 120(suppl. 1):71-83 (2012)

Storage/Stability

At least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Instructions for Use

See assay kit data sheet for detailed protocol.

Applications

1. Study enzyme kinetics
2. Screen for small molecular inhibitors for drug discovery and High Throughput (HTS) applications.

Shipping Temperature

dry ice

Warnings

Avoid freeze/thaw cycles.

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Neuroscience

Regulatory Status

Research Use Only

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