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STK33 Human, recombinant, N-terminal GST-tag Catalog #: 40093 Size: 10 µg Description: STK33 (GenBank Accession No. NM_030906.2), amino acids 2-end, with N-terminal GST-tag, MW = 84 kDa, expressed in a Baculovirus-infected Sf9 cell expression system.
Specific Activity: 2.8 pmol/min/μg. Assayed using 5 μg/ml STK33 in 10 mM Tris, pH 7.4, 5 mM MgCl2, 0.5 mM EDTA, 0.5 mM DTT, 5 μM ATP, and 400 μg/ml MBP substrate for 1 hr. at room temperature.
Application: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Formulated in: 25 mM Tris-HCl, pH 8.0,
100 mM NaCl, 0.05% Tween-20, 30% glycerol, and 3 mM DTT. Stability: >6 months at –80°C References: 1. Mujica, A.O., et al., 2001. Gene 280: 175- 181. 2. Brauksiepe, B., et al., 2008. Biochemistry 9: 25-37.
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