UbcH7 (UBE2G2)
Human, recombinant, N-terminal His-tag
Catalog #: 80317

Size: 100 µg

Description:  UbcH7,  also  known  as
UBE2G2  (GenBank  Accession  No.
NM_003347),  amino  acids  2-end,  with  Nterminal
 His-tag, MW  =  18  kDa,  expressed
in an E. coli cell expression system.
 
Activity Assay: 50  μl  reaction  mix  (4
mM ATP, 5 mM MgCl2, 1 mM DTT, UBE1
(0.5  μg),  UbcH7  or  other  E2,  Biotin-
Ubiquitin  (0.5 μg)  and Smurf2  (2 μg)  in 50
mM  Tris  pH  7.4)  for  30 min  at  37°C,  then
add  to  streptavidin  coated plates  for 30 min
at  37°C.  Incubate  with  anti-Smurf2,  then
Europium-labeled  secondary  antibody,  and
detect  by  time  resolved  fluorescence
(λex=330 nm, λem=620 nm).

Application:  Useful  for  the  study  of
enzyme  kinetics,  screening  inhibitors,  and
selectivity profiling.

Formulated in:  25  mM  Tris-HCl,  pH  8.0,
100 mM NaCl, 0.05% Tween-20, 20% glycerol,
and 3 mM DTT.
 
Stability: >6 months at –80°C 
 
References:
1. Katsanis, N., et al. 1998.  Genomics 51: 128-
131.
2. Cook, W.J. et al., 1997.  Biochemistry 36 (7):
1621–1627.