PKM2 mouse
Mouse, recombinant, N-terminal HIS tag
Catalog #: 50296

Size: 20 µg

Description: Mouse Pyruvate kinase M2
(PKM2) (GenBank Accession No.
NM_011099), amino acids 2-end, with
N-terminal His tag, MW = 58 kDa,
expressed in an E.Coli expression system.

Specific Activity: 11 pmol/min/μg.
A 50 μl PKM2 reaction is conducted in a
buffer containing 50 mM Tris (pH 7.4),
100mM MgCl2, 250 mM KCl, 40 mM ADP,
100 mM phosphoenolpyruvate (PEP) at
room temperature for 15 min. ATP
production is detected using Kinase-Glo
Luminescent Kinase Assay Platform.

Application: Useful for the study of
enzyme kinetics, screening inhibitors, and
selectivity profiling.

Formulated in: 25 mM Tris-HCl, pH 8.0,
100 mM NaCl, 0.05% Tween-20, 30% glycerol,
and 3 mM DTT.

Stability: >6 months at –80°C

References:
1. Spoden G.A. et al., Exp. Cell Res. 315 (16),
2765-2774 (2009).
2. Christofk H.R. et al., Nature 452 (7184), 230-
233 (2008).