SUV39H1 Full Length
Human, recombinant, N-terminal GST tag
Catalog #: 51071

Size: 5 µg

Description: Human SUV39H1
(GenBank Accession No. NM_003173), full
length (aa 2-end), with N-terminal GST tag,
MW= 74 kDa, expressed in a baculovirus-infected
Sf9 cell expression system.

Synonyms: KMT1A

Specific Activity: 76 pmol/min/mg
Assay conditions: 80 μl containing 50 mM
TRIS pH 8.8, 5 mM MgCl2, 4 mM DTT, 10 μM
substrate peptide, 10 μM S-adenosylmethionine,
and 0-50 nM SUV39H1 for 1 hr at 37ºC.
Then add 10 μl of fluorescently labeled tracer
and 10 μl of anti-Sadenosylhomocysteine
antibody, incubate 1 hr on a rotating platform.
Fluorescent polarization measured at room temperature
(λex=485 nm, λem=528 nm).

Application: Useful for the study of enzyme kinetics,
screening inhibitors, and selectivity profiling.

Formulated in: 25 mM Tris-HCl, pH 8.0,
130 mM NaCl, 0.05% Tween-20, 20% glycerol,
and 3 mM DTT

Stability: >6 months at –80°C

References:
1. Kang, M.Y. et al., Int. J. Cancer 121 (10),
2192-2197 (2007).
2. Spensberger, D. and Delwel, R. FEBS Lett.
582 (18), 2761-2767 (2008).