PDE1B-HEK293 Recombinant Cell line
Cat. # 60402

Product Description
Recombinant HEK293 cell line expressing human PDE1B (phosphodiesterase 1B ,
accession number NM_000924).

Format
Each vial contains 1 X 10^6 cells in 1 ml of 10% DMSO.

Introduction
PDE1B plays a role in signal transduction by regulating the intracellular concentration of
cyclic nucleotides. PDE1B hydrolyzes both cAMP and cGMP to nucleoside 5-prime
monophosphate. Although it prefers cGMP at low substrate levels it has a Vmax that is
approximately equal for both cGMP and cAMP (Polli JW and Koncaid RL, 1994; Yu J.
et al., 1997).

cAMP is an important signal carrier necessary for the proper biological response of cells
to hormones and other extracellular signals. It is required for cell communication in the
hypothalamus/pituitary gland axis and for the feedback control of hormones (Alewijnse
A.E. et al.,1997).

References
Polli, JW and Kincaid RL. (1994). “ Expression of a calmodulin-dependent
phosphodiesterase isoform (PDE1B1) correlates with brain regions having extensive
dopaminergic innervation.” J. Neurosc. 14 : 1251-1261.

Yu, J, et al. (1997). “Identification and Characterisation of a Human Calmodulin-
Stimulated Phosphodiesterase PDE1B1 “ Cell Signalling 9 (7): 519-529.
Bender A.T. et al., (2005). “ Selective up-regulation of PDE1B2 upon monocyte-tomacrophage
differentiation.” PNAS 102 (2): 497-502 .

Alewijnse, AE et al. (1997). “Modulation of forskolin-mediated adenylyl cyclase
activation by constitutively active Gs-coupled receptors.”. FEBS Letters 419 (2-3): 171-
174.

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