PRMT8, His-FLAG-tags Recombinant

Catalog #

51052

$450 *

Size: 20 µg

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Description

Human PRMT8 (protein arginine methyltransferase 8), GenBank Accession No. NM_019854, a.a. 61-394(end) with N-terminal His-FLAG-tag, MW=40.8 kDa, expressed in Sf9 cells using a Baculovirus expression system.

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Synonyms

protein arginine N-methyltransferase 8, PRMT8, Histone-arginine N-methyltransferase 8, HRMT1L3, HRMT1L4, PRMT-8

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Product Data Gallery

10% SDS-PAGE Coomassie Staining

Specific Activity

PRMT8 inhibition curve using a chemiluminescent assay (#52062). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥90%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 80 µg/ml FLAG peptide, 3 mM DTT, 20% glycerol.

40.8 kDa

Amino Acids

61–394(end)

Specific Activity

2.22 pmol/min/µg

Genbank #

NM_019854

UniProt #

Q9NR22

Tag(s)

N-terminal His-FLAG-tags

Background

Membrane-associated arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and asymmetrical dimethylarginine (aDMA). Able to mono- and dimethylate EWS protein; however its precise role toward EWS remains unclear as it still interacts with fully methylated EWS.

References

1. Bedford MT., et al. J Biol Chem. 2007 Dec 14;282(50):36444-53.
2. Taneda T., et al Brain Res. 2007 Jun 25;1155:1-9.

Storage/Stability

>6 months at -80°C.

Assay Conditions

50 µl reaction mix (20 mM phosphate buffer pH 7.4, 20 µM S-adenosyl methionine, and 1-5 ng methyltransferase PRMT8) added to the wells coated with the substrate. Incubate at RT for 1 hr. Then, add secondary HRP-labeled antibody and incubate 30 min. Finally,add HRP chemiluminescent substrates and read luminesence.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Methyltransferase

Regulatory Status

Research Use Only

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Datasheets/Protocols

51052_Lot110727.pdf 51052_Lot120416.pdf

Datasheets provide example lot-specific information. Contact us to request information on currently available lots.

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PRMT8, His-FLAG-tags Recombinant

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