The PRMT7 Homogeneous Assay Kit (Automethylation) is designed to measure PRMT7 automethylation for screening and profiling applications. Automethylation of PRMT7 plays a key role in inducing the epithelial–mesenchymal transition program and in promoting the migratory and invasive behavior of breast cancer cells. The key to the PRMT7 Homogeneous Assay Kit (Automethylation) is a highly specific antibody that recognizes methylated PRMT7. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, a sample containing PRMT7 enzyme is incubated with SAM. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts.
The PRMT7 Homogeneous Assay Kit (Automethylation) comes in a convenient AlphaLISA® format, with S-adenosylmethionine (SAM), primary antibody, methylation assay buffer, and purified Flag-tagged PRMT7 for 384 enzyme reactions.
AlphaScreen® Flag donor beads, 5 mg/ml (PerkinElmer #AS103D)
Optiplate-384 (PerkinElmer #6007290)
Dithiothreitol (DTT), 0.5 M
Format
UniProt #
Q9NVM4
References
1. You Feng, et al. 2014. J Biol Chem 289:32604. 2. Pengyu Geng, et al. 2017. FASEB J.31:2287.
Storage/Stability
At least 6 months from date of receipt when stored as directed.
Instructions for Use
See assay protocol for detailed instructions.
Shipping Temperature
-80°C
Contraindications
Green and blue dyes that absorb light in the AlphaScreen® signal emission range (520-620 nm), such as Trypan Blue. Avoid the use of the potent singlet oxygen quenchers such as sodium azide (NaN3) or metal ions (Fe2+, Fe3+, Cu2+, Zn2+ and Ni2+). The presence of >1% RPMI 1640 culture medium leads to a signal reduction due to the presence of excess biotin and iron in this medium. MEM, which lacks these components, does not affect AlphaScreen® assays.
Warnings
Avoid multiple freeze/thaw cycles.
Disclaimers and Limitations
BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.
End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.