PRMT7 Homogeneous Assay Kit (Automethylation)

Catalog #
79721
$1,125 *
Size: 384 Reactions
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Description

The PRMT7 Homogeneous Assay Kit (Automethylation) is designed to measure PRMT7 automethylation for screening and profiling applications. Automethylation of PRMT7 plays a key role in inducing the epithelial–mesenchymal transition program and in promoting the migratory and invasive behavior of breast cancer cells. The key to the PRMT7 Homogeneous Assay Kit (Automethylation) is a highly specific antibody that recognizes methylated PRMT7. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, a sample containing PRMT7 enzyme is incubated with SAM. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts.

Note: To study methylation of histone proteins by PRMT7, we recommend using our PRMT7 Homogeneous Assay Kit (H2b), #79720.

Need us to run inhibitor screens or profile your compounds against PRMT7 (Automethylation)? Check out our Methyltransferase Screening Services

Synonyms
Protein Arginine Methyltransferase 7, [Myelin Basic Protein]-Arginine N-Methyltransferase PRMT, Histone-Arginine N-Methyltransferase PRMT7, Protein Arginine N-Methyltransferase 7, EC 2.1.1.321, KIAA1933, EC 2.1.1, SBIDDS, PRMT-7
Product Info
Storage and Usage
Citations
Assay Kit Format
AlphaLISA®
Supplied As
The PRMT7 Homogeneous Assay Kit (Automethylation) comes in a convenient AlphaLISA® format, with S-adenosylmethionine (SAM), primary antibody, methylation assay buffer, and purified Flag-tagged PRMT7 for 384 enzyme reactions.
Materials Required But Not Supplied

AlphaLISA® anti-rIgG acceptor beads, 5 mg/ml (PerkinElmer #AL104C)

AlphaScreen® Flag donor beads, 5 mg/ml (PerkinElmer #AS103D)

Optiplate-384 (PerkinElmer #6007290)

Dithiothreitol (DTT), 0.5 M

Format

Assay Kit Components

UniProt #
Q9NVM4
References

1. You Feng, et al. 2014. J Biol Chem 289:32604.
2. Pengyu Geng, et al. 2017. FASEB J. 31:2287.