PRMT6 (HRMT1L6) Chemiluminescent Assay Kit
The PRMT6 Direct Activity Assay kit is designed to measure PRMT6 activity for screening and profiling applications. The key to the PRMT6 Direct Activity Assay Kit is a highly specific antibody that recognizes methylated R3 residue of Histone H4. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme for one hour. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.
| Catalog # | Component | Amount | Storage | |
| 51049 | PRMT6 human recombinant enzyme | 20 µg | -80°C | Avoid freeze/ thaw cycles |
| 52120 | 20 µM S-adenosylmethionine* | 250 µl | -80°C | |
| 52150 | Primary antibody 4 | 100 µl | -80°C | |
| 52131H | Secondary HRP-labeled antibody 2 | 10 µl | -80°C | |
| 52192 | 4x HMT assay buffer 6** | 3 ml | -20°C | |
| 52100 | Blocking buffer 4 | 50 ml | +4°C | |
| HRP chemiluminescent substrate A (transparent bottle | 6 ml | +4°C | ||
| HRP chemiluminescent substrate B (brown bottle) | 6 ml | +4°C | ||
| 96-well plate precoated with histone substrate | 1 plate | +4°C | ||
*Decreasing S-adenosylmethionine concentration will make the assay more sensitive to the inhibitors.
**Add 31 µl of 0.5 M DTT before use.
Yang, Y., Bedford, M.T. 2013. Nat Rev Cancer. 13(1):37-50.
Application Reference(s):
1. Peptidic transition state analogues as PRMT inhibitors, (2019)
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