PRMT6, His-tag Recombinant

Catalog #

51049

$330 *

Size: 20 µg

Qty

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Description

Human PRMT6 also known as HRMT1L6 and protein arginine N-methyltransferase 6, GenBank Accession No. NM_018137, a.a. 2-375(end), with N-terminal His-tag, expressed in an E. coli expression system. MW=43 kDa.

This product has been cited 1 time.

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Synonyms

PRMT6, HRMT1L6, protein arginine N-methyltransferase 6, PRMT-6

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

PRMT6 (HRMT1L6) inhibition curve using an AlphaLISA assay (#52056). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations1

Species

Human

Host Species/Expression System

E. coli

Purity

≥41%

Supplied As

Aqueous buffer solution.

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 200 mM imidazole, 20% glycerol, and 3 mM DTT.

43 kDa

Amino Acids

2–375(end)

Specific Activity

0.036 pmol/min/μg

Genbank #

NM_018137

UniProt #

Q96LA8

Tag(s)

N-terminal His-tag

Background

Arginine methyltransferase that can both catalyze the formation of monomethylarginine (MMA) and asymmetrical dimethylarginine (aDMA), with a strong preference for the formation of aDMA. Preferentially methylates arginyl residues present in a glycine and arginine-rich domain and displays preference for monomethylated substrates. Specifically mediates the asymmetric dimethylation of histone H3 Arg-2 to form H3R2me2a. H3R2me2a represents a specific tag for epigenetic transcriptional repression and is mutually exclusive with methylation on histone H3 Lys-4 (H3K4me2 and H3K4me3). It thereby acts as a transcription corepressor of various genes such as HOXA2. Also methylates histone H2A and H4 Arg-3 (H2AR3me and H4R3me, respectively). Acts as a regulator of DNA base excision during DNA repair by mediating the methylation of DNA polymerase beta (POLB), leading to stimulate the polymerase activity by enhancing DNA binding and processivity. Methylates HMGA1. May play a role in innate immunity against HIV-1 in case of infection by methylating and impairing the function of various HIV-1 proteins such as Tat, Rev and Nucleocapsid protein p7 (NC).

References

1. Hyllus D. et al., J. Genes Dev. 21(24), 3369-3380 (2007).
2. Guccione E. et al., Nature 449(7164), 933-937 (2007).

Storage/Stability

At least 6 months at -80°C.

Assay Conditions

For standard, asymmetric H4 R3(2Me)-Biotin was coated onto a neutravadin plate at varying concentrations. Biotin was coated onto a neutravadin plate at 0.4 μg/ml. Reaction mixture contained 50 mM Tris buffer pH 8, 10 mM NaCl, 1 mM DTT, 0.05% Tween-20, 40 μM S-adenosylmethionine, and various amounts of PRMT6—incubated for 1 hour at room temperature. Followed by 1-hour incubation with primary antibody against asymmetric H4 R3(2Me), and 30-minute incubation with secondary antibody. Luminescence was developed with HRP substrate A and B.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Methyltransferase

Regulatory Status

Research Use Only