Complex of human recombinant PRMT and MEP50 proteins. Recombinant human PRMT5 (protein arginine methyltransferase 5, variant 1), encompassing amino acids 2-637(end). This construct contains an N-terminal Flag-tag. Recombinant human MEP50 (WD repeat domain 77, variant 2), encompassing amino acids 2-342(end). This construct contains an N-terminal His-tag (6xHis). The two proteins were co-expressed and affinity purified.
Three types of arginine methylation exist in mammalian cells: monomethylarginine (MMA), asymmetric dimethylarginine (ADMA) and symmetric dimethylarginine (SDMA). Type I PRMTs ((CARM1, PRMT1, PRMT2, PRMT3, PRMT6, and PRMT8) catalyze ADMA, while type II PRMTs (e.g. PRMTs 5 and 7) catalyze SDMA. Type II PRMTs are found to be strongly implicated in diseases like cancer. For example, PRMT5 plays a role in the repression of certain tumor suppressor genes such as RB tumor suppressors while PRMT7 overexpression is observed in breast cancer
Assay was performed according to PRMT5 Chemiluminescent Assay Kit (BPS Bioscience #52002L) with PRMT5 (BPS Bioscience #51048) enzyme titrated from at various concentrations.
Instructions for Use
Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.
Applications
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.