PRMT5 TR-FRET Assay Kit

Catalog #

52171

$1,350 *

Size: 384 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The PRMT5 TR-FRET Assay Kit is designed to measure PRMT5 activity in a homogeneous 384 reaction format. PRMT5 is a histone methyltransferase that exhibits methylation activity toward H4-R3. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The key to the PRMT5 TR-FRET Assay Kit is a highly specific antibody that recognizes methylated substrate.

Need us to run inhibitor screens or profile your compounds against PRMT5? Check out our Methyltransferase Screening Services or DNA Replication & Repair Screening Services.

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Synonyms

PRMT5, HRMT1L5, IBP72, JBP1, SKB1, HTRF, PRMT-5

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Product Data Gallery

Product Info

Storage and Usage

Citations

Assay Kit Format

TR-FRET

Supplied As

The PRMT5 TR-FRET Assay Kit comes in a convenient format, with histone H4 peptide substrate, a Eu-labeled antibody, S-adenosylmethionine, methyltransferase assay buffer, FRET detection buffer, dye-labeled acceptor, and purified PRMT5.

Format

Catalog #	Component	Amount	Storage
51045	PRMT5/MEP50*	40 µg	-80°C	Avoid freeze/ thaw cycles!
52120	60 µM S-adenosylmethionine	250 µl	-80°C
	Eu-labeled antibody	5 µl	-80°C
	Biotinylated histone H4 peptide substrate	1000 rxns	-80°C
	4x PRMT5 assay buffer**	3 x 1 ml	-20°C
	Dye-labeled acceptor	2 x 10 µl	-20°C
78314	PRMT5 TR-FRET Detection Buffer	4 ml	-20°C
79969	White, nonbinding, low volume, microtiter plate	1	Room Temp.

*The concentration of PRMT5 is lot-specific and will be indicated on the tube containing the enzyme.
**Add 10 µl of 0.5 M DTT per vial before use.

Background

PRMT5 is a histone methyltransferase that exhibits methylation activity toward H4-R3. PRMT5 is a critical regulatory-protein linked to genome organization, cell cycle regulation, and stem cell differentiation. PRMT5 transfers methyl groups from S-adenosylmethoinine (SAM) to form mono and symmetric di-methylated arginine residues in histone proteins, transcription factors, and other regulatory proteins. Given its regulatory function, PRMT5 is believed to contribute to tumorigenesis. Its dysregulated activity has been associated with many cancers including lymphomas, lung, and breast cancer. Additionally, PRMT5 has been linked to other diseases including kidney and heart disease as well as neurological disorders such as Huntington’s and Alzheimer’s diseases.

References

Yang, Y., Bedford, M.T. 2013. Nat Rev Cancer. 13(1):37-50.

Storage/Stability

6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Instructions for Use

See assay kit data sheet for detailed protocol.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Methyltransferase

Regulatory Status

Research Use Only