PRMT5 Chemiluminescent Assay Kit

Catalog #

52002L

$865 *

Size: 96 reactions

Qty

Bulk/Custom

Add to Favorites

*US Pricing only. For international pricing, please contact your local distributor.

●

Purchase

●

Description

The PRMT5 Direct Activity Chemiluminescent Assay Kit is designed to measure PRMT5 activity for screening and profiling applications. The key to the PRMT5 Chemiluminescent Assay Kit is a highly specific antibody that recognizes methylated R3 residue of Histone H4. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme for one hour. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.

Need us to run inhibitor screens or profile your compounds against PRMT5? Check out our Methyltransferase Screening Services or DNA Replication & Repair Screening Services.

●

Synonyms

PRMT5, HRMT1L5, IBP72, JBP1, SKB1, PRMT-5

●

Product Data Gallery

Product Info

Storage and Usage

Citations

Assay Kit Format

Chemiluminescent

Supplied As

Kit comes in a convenient format, with a 96-well plate precoated with histone H4 peptide substrate, reagents, and purified PRMT5 enzyme for 100 enzyme reactions.

Format

Catalog Number	Components	Amount	Storage
51045	PRMT5/MEP50 human enzyme	20 µg	-80°C	Avoid freeze/ thaw cycles!
52120	100 µM S-adenosylmethionine	250 µl	-80°C
52150-3	Primary antibody 4-3	100 µl	-80°C
52131H	Secondary HRP-labeled antibody 2	10 µl	-80°C
	4x PRMT5 assay buffer (add DTT before experiment)	3 ml	-20°C
79556	Blocking buffer	50 ml	+4°C
	HRP chemiluminescent substrate A (transparent bottle)	6 ml	+4°C
	HRP chemiluminescent substrate B (brown bottle)	6 ml	+4°C
	96-well plate precoated with histone substrate	1	+4°C

*The concentration of PRMT5/MEP50 is lot-specific and will be indicated on the tube containing the enzyme.

UniProt #

O14744

Background

PRMT5 is a histone methyltransferase that exhibits methylation activity toward H4-R3.

References

Dillon S.C., et al. 2005. Genome Biology 6:227.

Storage/Stability

6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Instructions for Use

See assay kit data sheet for detailed protocol.

Applications

Useful for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.

Shipping Temperature

-80°C

Notes

Since May of 2017, this kit has been improved with a more specific antibody and reformulated buffers. The previous version of PRMT kit #52002L can still be purchased upon special request.

Contraindications

DMSO >1%, strong acids or bases, ionic detergents, high salt

Warnings

Avoid freeze/thaw cycles.

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Methyltransferase

Regulatory Status

Research Use Only