PRMT4 Homogeneous Assay Kit

Catalog #

52068

$2,050 *

Size: 384 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The PRMT4 Homogeneous Assay Kit is designed to measure PRMT4 activity for screening and profiling applications. PRMT4 (CARM1) is an arginine methyltransferase and dual functional coregulator that facilitates transcription initiation by methylation of Arg17 and Arg26 of histone H3. The PRMT4 Homogeneous Assay Kit comes in a convenient AlphaLISA® format, with His-tagged PRMT4 substrate, primary antibody, methylation assay buffer, and purified PRMT4 for 384 enzyme reactions. The key to the PRMT4 Homogeneous Assay Kit is a highly specific antibody that recognizes methylated substrate. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, a sample containing PRMT4 enzyme is incubated with the substrate. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts.

Need us to run inhibitor screens or profile your compounds against PRMT4? Check out our Methyltransferase Screening Services.

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Synonyms

PRMT4, CARM1, Coactivator-Associated Arginine Methyltransferase 1, PRMT-4

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Product Data Gallery

Product Info

Storage and Usage

Citations

Assay Kit Format

AlphaLISA®

Supplied As

Kit comes in a convenient AlphaLISA® format, with biotinylated histone H4 peptide substrate, primary antibody, methylation assay buffer, and purified PRMT5 for 384 enzyme reactions.

Materials Required But Not Supplied

AlphaLISA® anti-rIgG acceptor beads, 5 mg/ml (PerkinElmer #AL104C)
AlphaScreen Nickel donor beads, 5 mg/ml (PerkinElmer #AS101D)
Optiplate-384 (PerkinElmer #6007290)

Format

Catalog #	Component	Amount	Storage
51047	PRMT4/CARM1*	60 µg	-80°C	Avoid freeze/ thaw cycles!
52120	100 μM S-adenosylmethionine	250 µl	-80°C
52140Z3	Primary antibody 28	20 µl	-80°C
	PRMT4 substrate	300 µl	-80°C
	4x PRMT4 assay buffer (add 30 µl of 500 mM DTT before experiment)	3 ml	-20°C
	4x Detection buffer	2 ml	-20°C

*The concentration of PRMT4 is lot-specific and will be indicated on the tube containing the enzyme

UniProt #

Q86X55

References

Yang, Y., Bedford, M.T. 2013. Nat Rev Cancer. 13(1):37-50.

Storage/Stability

6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Instructions for Use

See assay kit data sheet for detailed protocol.

Applications

Useful for studying enzyme kinetics and HTS applications.

Shipping Temperature

-80°C

Notes

AlphaScreen^® and AlphaLISA^® are registered trademarks of PerkinElmer, Inc.

Contraindications

Green and blue dyes that absorb light in the AlphaScreen® signal emission range (520-620 nm), such as Trypan Blue. Avoid the use of the potent singlet oxygen quenchers such as sodium azide (NaN3) or metal ions (Fe2+, Fe3+, Cu2+, Zn2+ and Ni2+). The presence of >1% RPMI 1640 culture medium leads to a signal reduction due to the presence of excess biotin and iron in this medium. MEM, which lacks these components, does not affect AlphaScreen® assays.

Warnings

Avoid freeze/thaw cycles.

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Methyltransferase

Regulatory Status

Research Use Only

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PRMT4 Homogeneous Assay Kit

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