PRMT3, GST-tag Recombinant

Catalog #

51043

$330 *

Size: 50 µg

Qty

Bulk/Custom

Add to Favorites

*US Pricing only. For international pricing, please contact your local distributor.

●

Purchase

●

Description

Human PRMT3 (GenBank Accession No. NM_005788), a.a. 2-end, with N-terminal GST-tag, MW=86 kDa, expressed in an E. coli expression system.

This product has been cited 3 times.

●

Synonyms

PRMT3, HRMT1L3, PRMT-3

●

Product Data Gallery

10% SDS-PAGE Coomassie Staining

Specific Activity

PRMT3 inhibition curve using an AlphaLISA assay (#52055). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations3

Species

Human

Host Species/Expression System

E. coli

Purity

≥60%

Format

Aqueous buffer solution

Formulation

45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 18 mM glutathione, 10% glycerol, 3 mM DTT

86 kDa

Amino Acids

2–end

Specific Activity

490 pmol/min/µg

Genbank #

NM_005788

UniProt #

Q8WUV3

Tag(s)

N-terminal GST-tag

Background

PRMT3 is a protein arginine methyltransferase that catalyze the formation of asymmetric N(G),N(G)-dimethylarginine (ADMA) residues in proteins. 40S ribosomal protein S2 is a physiological substrate of PRMT3 and PRMT3 regulates ribosome biosynthesis at a stage beyond pre-rRNA processing. DAL-1 (differentially expressed in adenocarcinoma of the lung)/4.1B is a tumor suppressor gene that can significantly inhibits PRMT3 methylation of cellular substrates. Modulation of post-translational methylation may be an important mechanism through which DAL-1/4.1B affects tumor cell growth.

References

1. Choi S. et al., Biochim Biophys Acta. 2008 Sep,1780, 9, :1062-9.
2. Bachand F. and Silver P.A., EMBO J. 2004 Jul 7,23, 13, :2641-50.

Storage/Stability

>6 months at -80°C.

Assay Conditions

50 µl reaction mix (20 mM phosphate buffer pH 7.4, 20 µM S-adenosyl methionine, and 1-10 ng methyltransferase PRMT3) add to the wells coated with the substrate. Incubate at room temperature for 1 hr. Add antibody against methylated R3 residue of histone H4, incubate 1 hr. Add secondary HRP-labeled antibody and incubate 30 min. Finally, add HRP chemiluminescent substrates and read luminesence.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Methyltransferase

Regulatory Status

Research Use Only

Skip to the end of the images gallery

Skip to the beginning of the images gallery

●

Datasheets/Protocols

51043_Lot110912GC.pdf 51043_Lot110111.pdf

Datasheets provide example lot-specific information. Contact us to request information on currently available lots.

● General Protocols

● FAQs

● Contact Technical Service

PRMT3, GST-tag Recombinant

PRODUCTS

SERVICES

RESOURCES

COMPANY