PRMT1, His-GST-tag (Sf9-derived) Recombinant

Catalog #

51041

$330 *

Size: 20 µg

Qty

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This product has been cited 8 times.

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Synonyms

PRMT1, HRMT1L2, IR1B4, HCP1, PRMT-1

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations8

Species

Human

Construct

PRMT1(His-GST-2-371(end))

Host Species/Expression System

Sf9 insect cells

Purity

≥86%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20 and 20% glycerol

70 kDa

Amino Acids

2-371(end)

Genbank #

NM_001536

UniProt #

Q99873

Tag(s)

N-terminal His-GST-tag

Background

PRMT1 is a protein arginine methyltransferase that functions as a histone methyltransferase specific for histone H4. PRMT1 specifically methylates arginine-3 of histone H4 in vitro and in vivo. Methylation of arg3 by PRMT1 facilitates subsequent acetylation of histone H4 tails by p300. PRMT1 performs methylation of arg31 of STAT1 and this is required for transcription induced by IFN-alpha/IFN-beta. Arginine methylation of STAT1 is an additional posttranslational modification regulating transcription factor function, and alteration of arginine methylation might be responsible for the lack of interferon responsiveness observed in many malignancies. Type I PRMTs ((CARM1, PRMT1, PRMT2, PRMT3, PRMT6, and PRMT8) catalyze asymmetric dimethylarginine (ADMA), while type II PRMTs (e.g. PRMTs 5 and 7) catalyze SDMA. PRMT1 is the primary methyltransferase that deposits the ADMA mark, and it accounts for over 90% of this type of methylation.

Storage/Stability

At least 6 months at -80°C.

Assay Conditions

Enzyme was incubated with 0.25 µM of biotinylated H4 1-21 peptide with 1 µM AdoMet in HMT buffer 2A in a 10 µl reaction. 5 µl of Anti-Rabbit AlphaLISA acceptor beads (1:250) and 5 µl of Primary Antibody 4 (1:20) were added and incubation continued for 30 min. Streptavidin Donor beads (1:125) were added (10 µl) and mixture was further incubated for 15 min at room temperature before A-counts were measured.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Methyltransferase

Regulatory Status

Research Use Only