PRMT1, GST-tag (Sf9-derived)

Catalog #: 51041
Size: 20 µg
$320
Species: Human
Host Species: Sf9 insect cells
MW: 68 kDa
Genbank #: NM_001536
Tag(s): N-terminal GST-tag
a.a: 2–end
Background:
PRMT1 is a protein arginine methyltransferase that functions as a histone methyltransferase specific for histone H4. PRMT1 specifically methylates arginine-3 of histone H4 in vitro and in vivo. Methylation of arg3 by PRMT1 facilitates subsequent acetylation of histone H4 tails by p300. PRMT1 performs methylation of arg31 of STAT1 and this is required for transcription induced by IFN-alpha/IFN-beta. Arginine methylation of STAT1 is an additional posttranslational modification regulating transcription factor function, and alteration of arginine methylation might be responsible for the lack of interferon responsiveness observed in many malignancies. Type I PRMTs ((CARM1, PRMT1, PRMT2, PRMT3, PRMT6, and PRMT8) catalyze asymmetric dimethylarginine (ADMA), while type II PRMTs (e.g. PRMTs 5 and 7) catalyze SDMA. PRMT1 is the primary methyltransferase that deposits the ADMA mark, and it accounts for over 90% of this type of methylation.
Description:
Human PRMT1 (GenBank Accession No. NM_001536), a.a. 2-end, with N-terminal GST-tag, MW= 68 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
UniProt
Q99873
Synonym(s):
PRMT1, HRMT1L2, IR1B4, HCP1
Specific Activity:
6.2 pmol/min/µg
Assay Conditions:
50 µl reaction mix (20 mM phosphate buffer pH 7.4, 20 µM S-adenosyl methionine, and 1-5 ng methyltransferase PRMT1) add to the wells coated with the substrate. Incubate at room temperature for 1 hr. Add antibody against methylated R3 residue of histone H4, incubate 1 hr. Add secondary HRP-labeled antibody and incubate 30 min. Finally, add HRP chemiluminescent substrates and read luminesence.
Formulation:
40 mM Tris pH 8.0, 300 mM NaCl, 20% glycerol and 1 mM DTT.
Format:
Aqueous buffer solution
Storage / Stability:

>6 months at -80°C.

Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s):
Warning(s):
Avoid freeze/thaw cycles
Scientific Category:
Methyltransferase
Product Type:
Recombinant Protein
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Product Datasheets

51041.pdf