PRMT1, GST-Tag (E. coli-derived) Recombinant

Catalog #
51040
$330 *
Size: 50 µg
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Description

Recombinant Human PRMT1 (protein arginine N-methyltransferase 1), encompassing a.a. 2- 371 (end). This construct contains an N-terminal GST-tag followed by a thrombin cleavage site. The protein was affinity purified.

 

Synonyms
PRMT1, HRMT1L2, IR1B4, HCP1, PRMT-1
Product Info
Storage and Usage
Citations
Species
Human
Host Species/Expression System
E. coli
Purity

≥80%

Format
Aqueous buffer solution
Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol, and 3 mM DTT

MW
69 kDa
Amino Acids
2-371(end)
Genbank #
NM_001536
UniProt #
Q99873
Tag(s)
N-terminal GST-tag
Background
PRMT1 is a protein arginine methyltransferase that functions as a histone methyltransferase specific for histone H4. PRMT1 specifically methylates arginine-3 of histone H4 in vitro and in vivo. Methylation of arg3 by PRMT1 facilitates subsequent acetylation of histone H4 tails by p300. PRMT1 performs methylation of arg31 of STAT1 and this is required for transcription induced by IFN-alpha/IFN-beta. Arginine methylation of STAT1 is an additional posttranslational modification regulating transcription factor function, and alteration of arginine methylation might be responsible for the lack of interferon responsiveness observed in many malignancies. Type I PRMTs ((CARM1, PRMT1, PRMT2, PRMT3, PRMT6, and PRMT8) catalyze asymmetric dimethylarginine (ADMA), while type II PRMTs (e.g. PRMTs 5 and 7) catalyze SDMA. PRMT1 is the primary methyltransferase that deposits the ADMA mark, and it accounts for over 90% of this type of methylation.
References