PRL3 (2-173), His-tag

Catalog #: 30002
Size: 50 µg
$375
Species: Human
Host Species: E. coli
MW: 19.5 kDa
Genbank #: NM_032611
Tag(s): N-terminal His-tag
a.a: 2–173
Background:
Protein tyrosine phosphatase which stimulates progression from G1 into S phase during mitosis. Enhances cell proliferation, cell motility and invasive activity, and promotes cancer metastasis. May be involved in the progression of cardiac hypertrophy by inhibiting intracellular calcium mobilization in response to angiotensin II.
Description:
Human PRL 3 (GenBank Accession No. NM_032611), a.a. 2-173 with N-terminal His-tag, MW = 19.5 kDa, expressed in an E. coli expression system.
Synonym(s):
PRL-3, PTP4A3, PTPIVa, PTPCAAX3, Phosphatase Of Regenerating Liver 3
Specific Activity:
0.53 pmol/min/µg
Assay Conditions:
Assay conditions: enzyme was incubated in 50 mM Tris pH 7.4, 150 mM NaCl, 5 mM DTT, and 12.5 mM PNPP as a substrate for 2 hours at 37°C. Absorbance was read by Tecan spectraphotometer at 405 nm wavelength.
Formulation:
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2mM KCl, 20% glycerol, and 3 mM DTT.
Format:
Aqueous buffer solution
Storage / Stability:

>12 months at -80°C.

Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s):
1. Dai N. et al., World J. Gastroenterol. 15 (12), 1499-1505 (2009).
2. Radke I. et al., Br. J. Cancer 95 (3), 347-354 (2006).
Warning(s):
Avoid freeze/thaw cycles
Scientific Category:
PTP/Non-rec.
Product Type:
Recombinant Protein
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Product Datasheets

30002_Lot130220G.pdf