PRL1 (2-173), His-tag

Catalog #: 30000
Size: 50 µg
$375
Species: Human
Host Species: E. coli
MW: 19.8 kDa
Genbank #: BC023975
Tag(s): N-terminal His-tag
a.a: 2–173
Background:
Protein tyrosine phosphatase which stimulates progression from G1 into S phase during mitosis. May play a role in the development and maintenance of differentiating epithelial tissues. Enhances cell proliferation, cell motility and invasive activity, and promotes cancer metastasis.
Description:
Human PRL 1 (GenBank Accession No. BC023975), a.a. 2-173 with N-terminal His-tag, MW = 19.8 kDa, expressed in an E. coli expression system.
Synonym(s):
PRL-1, PTP4A1, PTPIVa, PTPCAAX1, Phosphatase Of Regenerating Liver 1
Specific Activity:
0.168 pmol/min/µg
Assay Conditions:
Assay conditions: enzyme was incubated in 50 mM Tris pH 7.4, 150 mM NaCl, 5 mM DTT, and 12.5 mM PNPP as a substrate for 2 hours at 37°C. Absorbance was read by Tecan spectraphotometer at 405 nm wavelength.
Formulation:
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 200 mM imidazole
Format:
Aqueous buffer solution
Storage / Stability:

>12 months at -80°C.

Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s):
1. Luo Y. et al., Biochemistry. 2009. 48(8): 1838-1846
2. Sun J.P. et al., J Biol Chem. 2007 Sep 28,282(39):29043-51.
Warning(s):
Avoid freeze/thaw cycles
Scientific Category:
PTP/Non-rec.
Product Type:
Recombinant Protein
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Product Datasheets

30000_Lot90610.pdf