PRL1 (2-173), His-tag Recombinant

Catalog #

30000

$325 *

Size: 50 µg

Qty

Bulk/Custom

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Description

Human PRL 1 (GenBank Accession No. BC023975), a.a. 2-173 with N-terminal His-tag, MW = 19.8 kDa, expressed in an E. coli expression system.

This product has been cited 3 times.

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Synonyms

PRL-1, PTP4A1, PTPIVa, PTPCAAX1, Phosphatase Of Regenerating Liver 1

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations3

Species

Human

Host Species/Expression System

E. coli

Purity

≥75%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 200 mM imidazole

19.8 kDa

Amino Acids

2–173

Specific Activity

0.168 pmol/min/µg

Genbank #

BC023975

UniProt #

Q93096

Tag(s)

N-terminal His-tag

Background

Protein tyrosine phosphatase which stimulates progression from G1 into S phase during mitosis. May play a role in the development and maintenance of differentiating epithelial tissues. Enhances cell proliferation, cell motility and invasive activity, and promotes cancer metastasis.

References

1. Luo Y. et al., Biochemistry. 2009. 48(8): 1838-1846
2. Sun J.P. et al., J Biol Chem. 2007 Sep 28,282(39):29043-51.

Storage/Stability

>12 months at -80°C.

Assay Conditions

Assay conditions: enzyme was incubated in 50 mM Tris pH 7.4, 150 mM NaCl, 5 mM DTT, and 12.5 mM PNPP as a substrate for 2 hours at 37°C. Absorbance was read by Tecan spectraphotometer at 405 nm wavelength.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

PTP/Non-rec.

Regulatory Status

Research Use Only