PRL2 (2-167), His-tag

Catalog #: 30001
Size: 50 µg
$375
Species: Human
Host Species: E. coli
MW: 19.1 kDa
Genbank #: NM_080391
Tag(s): N-terminal His-tag
a.a: 2–167
Background:
Protein tyrosine phosphatase which stimulates progression from G1 into S phase during mitosis. Promotes tumors. Inhibits geranylgeranyl transferase type II activity by blocking the association between RABGGTA and RABGGTB.
Description:
Human PRL 2 (GenBank Accession No. NM_080391), a.a. 2-167 with N-terminal His-tag, MW = 19.1 kDa, expressed in an E. coli expression system.
Synonym(s):
PRL-2, PTP4A2, PTPIVa, PTPCAAX2, OV-1, Phosphatase Of Regenerating Liver 2
Specific Activity:
0.334 pmol/min/µg
Assay Conditions:
Assay conditions: enzyme was incubated in 50 mM Tris pH 7.4, 150 mM NaCl, 5 mM DTT, and 12.5 mM PNPP as a substrate for 2 hours at 37°C. Absorbance was read by Tecan spectraphotometer at 405 nm wavelength.
Formulation:
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2mM KCl, 20% glycerol, and 3 mM DTT.
Format:
Aqueous buffer solution
Storage / Stability:

>12 months at -80°C.

Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s):
1. Dumaual C.M. et al., J. Histochem. Cytochem. 54 (12), 1401-1412 (2006).
2. Radke I. et al., Br. J. Cancer 95 (3), 347-354 (2006).
Warning(s):
Avoid freeze/thaw cycles
Scientific Category:
PTP/Non-rec.
Product Type:
Recombinant Protein
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Product Datasheets

30001_Lot90604.pdf