PRDM9 Chemiluminescent Assay Kit

Catalog #

79625

$765 *

Size: 96 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The PRDM9 Chemiluminescent Assay Kit is designed to measure PRDM9 activity for screening and profiling applications, using purified PRDM9. The PRDM9 Chemiluminescent Assay Kit comes in a convenient format, with 8-well strips pre-coated with histone H3 peptide substrate, an antibody against methylated lysine on Histone H3, a secondary HRP-labeled antibody, S-adenosylmethionine, methyltransferase assay buffer, and enough purified PRDM9 enzyme for 100 enzyme reactions. The key to the PRDM9 Chemiluminescent Assay Kit is a highly specific antibody that recognizes methylated K36 residue of Histone H3. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme. Next, primary antibody is added. Finally, the plates are treated with an HRP-labeled secondary antibody followed by the addition of the HRP substrate to produce chemiluminescence that can be measured using a chemiluminescence reader.

Need us to run inhibitor screens or profile your compounds against PRDM9? Check out our Methyltransferase Screening Services or DNA Replication & Repair Screening Services.

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Synonyms

PRDM9, PR domain zinc finger protein 9, MEISETZ, PR/SET domain 9

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Product Data Gallery

Product Info

Storage and Usage

Citations

Assay Kit Format

Chemiluminescent

Supplied As

See description.

Materials Required But Not Supplied

• TBST buffer (1 x TBS, pH 8.0, containing 0.05% Tween-20)
• 0.5 M DTT (dithiothreitol) in anhydrous DMSO
• Luminometer or microplate reader capable of reading chemiluminescence
• Adjustable micropipettor and sterile tips
• Rotating or rocker platform

Format

UniProt #

Q9NQV7

References

Powers NR, et al. PLoS Genet. 2016 Jun 30; 12(6):e1006146.

Storage/Stability

Up to 6 months from date of receipt when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Instructions for Use

See assay kit data sheet for detailed protocol.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Methyltransferase

Regulatory Status

Research Use Only

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PRDM9 Chemiluminescent Assay Kit

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